摘要
为揭示福州左海湖细菌群落的季节变化及其与环境因子的关系,通过构建16SrRNA基因克隆文库对全湖水体细菌群落进行了调查,应用典范对应分析(Canonical correspondence analysis,CCA)探讨影响细菌群落季节性变化的环境因素。结果表明:左海湖细菌群落组成中β-变形菌门β-Proteobacteria和蓝细菌门Cyanobacteria为优势类群,α-变形菌门α-Proteobacteria、拟杆菌门Bacteroidetes和放线菌门Actinobacteria为亚优势类群。细菌群落结构具有明显的季节性变化特征,春、夏季Cyanobacteria为优势类群,秋、冬季β-Proteobacteria为优势类群。春季水体细菌群落结构主要受pH、叶绿素a(Chla)、透明度(SD)和总氮(TN)的影响,夏季水体受pH、Chla、SD、总磷(TP)影响较大,秋季水体受TP、Chla、TN影响较大,冬季水体则受水温(WT)、pH、SD影响较大。本研究发现,左海湖细菌群落结构的季节动态与水温及营养状况密切相关。
To determine the seasonal changes on and the environmental factors that affected the bacterial community at Zuohai Lake in Fuzhou,a survey was conducted at 4sampling sites on the lake from February to August in 2012.A genetic library of their 16 SrRNA gene clones was established.Canonical correspondence analysis（CCA）was used to examine the correlation between the bacterial diversity and the environmental factors.The results indicated thatβ-Proteobacteria and Cyanobacteria were the predominate lineages in the water,followed byα-Proteobacteria,Bacteroidetes,and Actinobacteria.A significant seasonal variation in composition of the bacterial community was detected.Among various categories of bacteria,Cyanobacteriaappeared most dominant in the spring and summer;whileβ-Proteobacteria,in the autumn and winter.The results of CCA showed that the bacterial community in the water was mainly affected by pH,chlorophyll a（Chla）,transparency（SD）,and total nitrogen（TN）in the spring;pH,Chla,SD,and total phosphate（TP）,in the summer;TP,Chla,and TN in the fall;and,water temperature,pH,and SD,in the winter.Thus,in general,the bacterial growth in the lake closely related to the temperature as well as the nutrients available in the water.
出处
《福建农业学报》
CAS
北大核心
2016年第7期758-764,共7页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(2011J01118)
福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1025-9)
