摘要
为了研究松花湖水体中微生物群落结构的多样性,采用聚合酶链式反应-变性凝胶梯度电泳(PCR-DGGE)对水体垂向空间分布的细菌进行了多样性分析。把每一个条带看作一个分类操作单位(OUT),表明水体细菌的微生物群落结构随着湖泊深度变化呈现显著的差异,其中S1和S2位点微生物群落单元(OUT)数高于其他位点。UPGMA聚类分析DGGE指纹图谱表明,水体不同深度的微生物群落结构之间相似性和动态性存在明显差异。微生物群落单元数与环境因子之间响应关系的典型对应分析(CCA)证实,水深(SH)、pH、温度(Wt)和溶解氧(DO)等环境因子与微生物群落多样性指标之间存在显著相关性,水深(SH)、温度(Wt)的变化是微生物群落结构变化的主要原因。
In order to study the diversity of microbial community structure in Songhua Lake, PCR-DGGE （Polymerase chain reaction-dena- turing gradient gel electrophoresis） technology is employed to study the bacterial community structure in the different spatial distribution. Each strip as a operational taxonomic unit（OUT）, suggested that bacterial microbial communities with water depth change will have a signifi- cant difference, microbial community unit （OUT） numbers higher than other sites in St and $2. The Unweighted pair group method with arithmetic averages （UPGMA） dendrogram of sample basis on DGGE fingerprints showed water microbial community diversity presents sig- nificant spatial difference, there have similar and dynamic in the different depths of water. Canonical correspondence analysis（CCA） was ap- plied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes sug- gested that the bacterial community composition was primarily correlated with the variables of depth of water（SH ）, temperature （Wt）, and dissolved oxygen（ DO ）. The change of depth of water（SH ） and temperature （Wt） were the main reason for the change of microbial community structure.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2013年第4期764-770,共7页
Journal of Agro-Environment Science
基金
国家水体污染控制与治理科技重大专项(2009ZX07106-001)
