摘要
以越冬期间库尔勒香梨花芽、树干、枝条等器官为材料,测定了不同器官形成层组织抗氧化酶(SOD、CAT、POD)活性、可溶性蛋白含量、相对电导率等抗寒指标,同时监测了果园气温和树干阳阴面形成层温度,并采用隶属函数评价不同器官抗寒性,分析越冬期气温对新疆库尔勒香梨不同器官抗寒指标和抗寒性的影响。结果表明:(1)在库尔勒香梨越冬期间,不同器官同一抗寒指标和同一器官不同抗寒指标随气温、树干阳阴面形成层温度差的变化趋势有所不同,但总体上抗寒指标均与气温呈负相关关系,而与树干阳阴面形成层温度差均呈正相关关系。(2)各器官形成层可溶性蛋白含量与其抗氧化酶活性呈正相关关系,SOD、CAT活性与POD活性呈正相关关系,并以CAT与POD活性相关性最强。(3)利用隶属函数法综合分析结果显示,贡献率最大的是CAT活性和可溶性蛋白含量,不同器官抗寒性强弱顺序为花芽组织>树干阴面形成层>枝条形成层>树干阳面形成层。研究发现,库尔勒香梨各器官形成层POD和CAT活性在越冬期间较低气温和较强烈形成层温度变化时较高,并以SOD对低温伤害和形成层温度变化最敏感;整个越冬期抗寒性强的器官表现出较高的可溶性蛋白含量和较低的相对电导率;形成层温度变化与抗寒生理指标变化之间具有较好的相关性,在一定范围内能够反映机体受到的低温冻害程度。
During over wintering period ,with the flower bud ,trunk cambium and branch as material ,we determined the cold hardiness indexes ,antioxidant enzyme activities （SOD ,CAT ,POD） ,soluble protein content and relative conductivity ,of different organs and tissues ,simultaneously monitored the orchard air temperature and trunk cambium temperature ,evaluated the cold resistance of different organs by using＆amp;nbsp;membership function method ,analyzed the effect of air temperature on cold hardiness indexes and cold re-sistance of Xinjiang Korla fragrant pear＇s different organs .Results showed that ：（1） There was a differ-ence between the changing trend of different organs same cold hardiness indexes and the same organ differ-ent cold hardiness indexes with the changes of air temperature ,but generally the air temperature and cold hardiness indexes were negatively correlated ;the cambium temperature difference and cold hardiness inde-xes were positively correlated .（2） Soluble protein content and cold hardiness indexes were positively cor-related ;SOD ,CA T and POD activities are positively correlated ,among them CA T and POD had relatively good correlation level .（3） While comprehensively evaluated the cold hardiness of different organs by using subordination function method CAT activity and soluble protein content had the biggest contribution .The cold hardiness sequence of different organs was flower bud〉 north-unexposed cambium 〉branch〉 south-exposed cambium .It was found that the POD and CAT activities expressed relatively high under the condi-tion of relatively low temperature and intensively cambium temperature changes ,SOD was the most sensi-tive protective enzyme to cambium temperature changes and freezing injury ;during whole winter ,the or-gan with the strong cold resistance contained relatively higher soluble protein content and lower relative conductivity ;there was relatively well correlation between the changes of cambium temperature and cold hardiness indexes .Under certain condition it can indicate the certain organism cold tolerance .
作者
艾克来木.艾合买提
阿不来提.买买提
巴特尔.巴克
阿丽腾.吐尔孙哈力
萨吉旦.阿卜杜克日木
祖力克艳.麻那甫
AKREM Ahmet ABLET Mamat BATUR Bake ALTUN Tursunhali SAJIDA Abdukarim ZULKIYE Manaf(College of Grassland and Environmental Science, Xinjiang Agricultural University, Urumqi 830052,China Eorest Management Station, Hotan Prefecture, Hotan, Xinjiang 848000, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2016年第7期1408-1416,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31260289)
新疆维吾尔自治区土壤学重点学科
关键词
越冬期
库尔勒香梨
器官
生理指标
抗寒性
over-wintering period
Korla fragrant pear
organ
physiological indexes
cold tolerance
