摘要
为获知盐度—光照强度—温度对半咸水硅藻——角毛藻SHOU-B98生长及高不饱和脂肪酸含量的影响,实验采用L_9(3~4)正交设计探讨其在不同盐度(6、12和18)、光照强度[10、30和50μmol/(m^2·s)]及温度(10、20和30℃)组合条件下的生长及藻细胞ARA、EPA和DHA含量的变化。结果显示,盐度、光照强度、温度三因素对角毛藻SHOUB98的生长及高不饱和脂肪酸含量均有显著影响,且存在因素间的交互作用。角毛藻SHOU-B98在盐度12、光照强度30μmol/(m^2·s)和温度30℃及盐度18、光照强度50μmol/(m^2·s)和温度20℃的组合条件下生长最快,第4天后这2组做藻的生物量显著高于其他组(P<0.05)。角毛藻细胞积累不同高不饱和脂肪酸的最佳培养条件也不同,细胞积累ARA的最优培养组合条件为盐度6、光照强度50μmol/(m^2·s)和温度30℃;细胞积累EPA和DHA的最优培养组合条件为盐度18、光照强度50μmol/(m^2·s)和温度20℃。研究表明,角毛藻SHOU-B98生长快且细胞脂肪酸营养价值高的培养条件为盐度18、光照强度50μmol/(m^2·s)和温度20℃的组合。
Orthogonal experiments L_9（3-4） were applied to investigate the effects of different salinity（6,12 and 18）,light intensity[10,30 and 50 μmol/（m-2·s）]and temperature（10,20 and 30℃） combinations on the growth and cellular contents of arachidonic acid（ARA）,eicosapentaenoic acid（EPA） and docosahexaenoic acid（DHA） in Chaetoceros sp.SHOU-B98.The results showed that salinity,light intensity,temperature and the interaction of the three factors had significant effects on growth and highly unsaturated fatty acid contents of the microalgae.Under salinity 12,light intensity 30 μmol/（m-2·s） and temperature 30 ℃ combination and salinity 18,light intensity50 μmol/（m-2·s） and temperature 20℃ combination,the microalgae displayed the fastest growth and highest biomass since the 4th day（P〈0.05）.The best culture conditions for cell to accumulate different highly unsaturated fatty acids were also different.The optimal combined culture condition for Chaetoceros sp.SHOU-B98 cell to accumulate ARA was combination of salinity 6,light intensity 50 μmol/（m-2·s） and temperature 30℃.While the optimal culture condition to accumulate EPA or DHA was combination of salinity 18,light intensity 50μmol/（m-2·s） and temperature 20℃.Therefore the suitable culture condition for microalgae Chaetoceros sp.SHOU-B98 with fast growth and high fatty acid nutrition value is the combination of salinity 18,light intensity 50μmol/（m-2·s） and temperature 20℃.
作者
黄旭雄
曾蓓蓓
穆亮亮
蔡敬
危立坤
HUANG Xuxiong ZENG Beibei MU Liangliang CAI Jing WEI Likun(Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China Aquatic Animal Genetic Breeding Center Collaborative Innovation Center in Shanghai, Shanghai Ocean University, Shanghai 201306, China)
出处
《水产学报》
CAS
CSCD
北大核心
2016年第9期1451-1461,共11页
Journal of Fisheries of China
基金
上海市科技兴农项目[沪农科攻字(2015)第1-2号]
上海市科技兴农项目[沪农科推字(2013)第2-1号]
上海高校水产学一流学科建设项目~~
关键词
角毛藻
盐度
光照强度
温度
高不饱和脂肪酸
Chaetoceros sp.
salinity
light intensity
temperature
highly unsaturated fatty acids
