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苦瓜a-苦瓜素基因启动子的克隆和序列分析

Cloning and Analysis of α-momorcharin Gene Promoter From Momordica chanrantia L.
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摘要 以苦瓜嫩叶为试材,采用染色体步移法,克隆了α-苦瓜素基因启动子序列,并对其进行序列分析。结果表明:该启动子长1.2kb,含有2个TATA box,2个CAAT box,以及参与茉莉酸甲酯代谢、防御和压力反应、热胁迫反应、光反应调节、昼夜节律调节等的顺式作用元件。 Taking tender leaf of M.charantiaas material,a total of 1.2kb alpha-momorcharin gene promoter was cloned by chromosome walking.The results showed that two TATA boxes,two CAAT boxes,and elements involving in jasmine acid methyl ester metabolism,defense,stress response,heat stress response,light response regulator,circadian rhythm regulation,had been identified.
作者 李志良 郑壮 杨谷良 向福 王书珍 LI Zhiliang ZHENG Zhuang YANG Guliang XIANG Fu WANG Shuzhen(College of Life Science, Huanggang Normal University, Huanggang, Hubei 43800)
出处 《北方园艺》 CAS 北大核心 2016年第18期99-102,共4页 Northern Horticulture
基金 黄冈师范学院科学研究资助项目(2014022203) 黄冈师范学院发展规划处资助项目(xfg2015C22) 大别山特色资源开发湖北省协同创新中心团队资助项目(2015TD07)
关键词 苦瓜 α-苦瓜素 染色体步移 启动子 Momordica charantia L. α-momorcharin chromosome walking promoter
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