摘要
目的:研究五虎汤对病毒诱发幼年哮喘大鼠脾细胞悬液T-bet、GATA-3、RORγt、Foxp3 m RNA表达的影响。方法:采用RSV诱导OVA致敏大鼠哮喘模型。造模成功后,随机分成五虎汤高、中、低[4.752 g/(kg·d)、2.376 g/(kg·d)、1.188 g/(kg·d)]剂量组、地塞米松组[地塞米松片,2.4 mg/(kg·d))]、模型组。干预14 d后,取脾组织,采用RT-PCR法检测脾细胞悬液中T-bet、GATA-3、RORγt、Foxp3 m RNA表达情况。结果:模型组脾组织T-bet、Foxp3m RNA表达低于空白组(P<0.05),而GATA-3、RORγt m RNA表达高于空白对照组(P<0.05)。五虎汤高、中剂量组脾组织GATA-3、RORγt m RNA表达均明显低于模型组(P<0.01或P<0.05),而T-bet、Foxp3 m RNA表达均明显高于模型组(P<0.01或P<0.05)。结论:五虎汤能调控病毒诱发幼年哮喘大鼠脾组织转录因子T-bet、GATA-3、RORγt、Foxp3 m RNA的表达,调控Thl、Th2、Thl7、Treg细胞,重建免疫平衡,抑制哮喘气道炎症形成。
Objective： To explore the effect of Wuhu Decoction on T-bet, GATA-3, RORγt and Foxp3 mRNA of spleen in young rats with virus induced asthma. Methods： The rat asthma model was established by the respiratory tract virus induced ovalbumin. The rats were randomly divided into six groups, the high, medium and low groups [4.752, 2.376 and 1.188 g/（kg·d）] of Wubu Decoction, the dexamethasone group （2.4 mg/（kg·d））, and the model group, after the model were established successfully. The expressive levels of T-bet, GATA-3, RORγt and Foxp3 mRNA of spleen in young rats with virus induced asthma were measured by western blot, after treatment for 14 days. Results： The model group showed lower expressive levels of T-bet and Foxp3 mRNA of spleen than the control group （P〈0.05）, while higher expressive levels of GATA-3 and RORγt mRNA of spleen than the control group （P〈0.05）. The high and medium groups of Wuhu Decoction showed lower levels of GATA-3 and RORγt mRNA in spleen than the model group （P〈0.01 or P〈0.05）, and higher levels of T-bet and Foxp3 mRNA in lung and spleen than the control group （P〈0.01 or P〈0.05）. Conclusion： Wuhu Decoction can inhibit GATA-3 and RORγt mRNA of spleen and promote T-bet and Foxp3 mRNA of lung and spleen, which function is regulating to the balance of Th1/Th2 and Th17/Treg.
出处
《中医药导报》
2016年第19期18-22,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省自然科学基金项目(No.13JJ3105)
