摘要
蓝耳病是全球生猪规模化养殖场所面临的共同难题,现仍缺少有效的治疗药物,而疫苗免疫是控制该病最为有效的手段。为了解粤北地区规模化猪场的蓝耳病抗体水平,对该地区4家猪场382份血清样品进行ELISA检测,并设计荧光定量RT-PCR方法对血清中的病毒RNA进行检测,以初步探讨猪群抗体水平与病毒血症的关系。结果显示:粤北地区规模化猪场的蓝耳病抗体阳性率为82.9%,平均S/P值为1.59;但抗体水平离散度较大,4家猪场抗体水平的变异系数均高于25%。经灵敏度为10-1.5 TCID50/m L的荧光定量RT-PCR方法检测显示,猪群病毒血症发生率为12.4%,其中抗体水平不稳定群中病毒血症发生率为16.7%,明显高于抗体稳定群的7.3%。研究表明,粤北地区地区抗体阳性率和抗体效价较高,但均一性差,蓝耳病发病的可能性较大;同时抗体不稳定猪群出现病毒血症的概率更高。
Porcine reproductive and respiratory syndrome （ PRRS ） is a serious problem common to the swine industry worldwide. Because it is lack of effective drug for treatment, vaccination is still the most effective means for preventing and controlling PRRS. In order to monitor the PRRSV antibody level of the scale pig farms in northern Guangdong, this study conducted ELISA of the PRRSV antibody of 382 serum samples from 4 pig farms in northern Guangdong. For explorating the relationship of PRRS antibody level and viremia, this study detected PRRSV RNA by fluorescence quantitative reverse-transcriptase PCR assay which was established in this study. Results showed that the positive rate of PRRS antibody of 4 pig farms was 82.9%. Mean value of S/P was 1.59. But the degree of dispersion was large, variable coefficient of S/P value was over 25% in all farms. This study found that the incidence of viremia of 4 pig farms was 12.4%, using the fluorescence quantitative RT-PCR whose detection limit was 10-15 TCID50/mL, and the incidence of viremia of antibody unstable group was 16.7%, significantly higher than antibody stable group, whose incidence was 7.3%. The positive rate and antibody titer in northern Guangdong were high, but consistency was poor, leading to high incidence of PRRS. Besides, this study also found that the incidence of viremia in antibody unstable groups was higher than that in stable groups.
出处
《广东农业科学》
CAS
2016年第8期143-150,共8页
Guangdong Agricultural Sciences
基金
广东省现代农业产业技术体系建设专项(生猪产业创新团队)
韶关市科技计划项目(2013CX/NO9
2014CX/N319)
韶关学院科研项目(314-140694)
