摘要
建立高效液相色谱(HPLC)法对川西藏区天然野樱桃酒中有机酸的种类和含量的检测方法。采用Aminex HPX-87H离子排阻色谱柱,以7 mmol/L H_2SO_4溶液(pH 2.20)为流动相,流速为0.6 m L/min,柱温60℃,紫外检测器检测波长为210 nm。经过方法学验证,该检测方法重现性好、精密度及准确度高,在0.001~2.000 g/L线性范围内线性关系良好(R^2>0.99),检出限为0.000 6 g/L,加标回收率为86%~106%,重现性试验、精密度试验和回收率试验的相对标准偏差(RSD)均<5%,适用于天然野樱桃酒中6种重要有机酸的同时检测。结果表明,川西藏区天然野樱桃酒样品中含量最高的有机酸是草酸(1.92 g/L)和L-苹果酸(0.78 g/L),其次是乙酸(0.17 g/L)和酒石酸(0.18 g/L),乳酸(0.15 g/L)和柠檬酸(0.15 g/L)含量最少。
A HPLC method for determination of different kinds and contents of organic acids in natural wild cherry wine from western Sichuan Tibetan areas was established. Using Aminex HPX-87H ion exclusion chromatography column, 7 mmol/L H2SO4 as mobile phase, flow rate 0.6 ml/min, column temperature 60℃ and detection wavelength 210 nm, the organic acids was determined by HPLC. Through the methodology validation, the detection method had good reproducibility, high precision and accuracy. The method had a good linear relationship in the range of 0.001-2.000 g/L, the limit of determination was 0.000 6 g/L, the adding standard recovery rate was 86%-106%, the relative standard deviation of reproducibility, precision and recovery rate experiments was all less than 5%, which indicated that the method was suitable for simultaneous detection of six kinds of important organic acids in natural wild cherry wine. The results showed that the highest contents of organic acids in natural wild cherry wine from westem Sichuan Tibetan areas were oxalic acid （1.92 g/L） and L-malic acid （0.78 g/L）, followed by acetic acid （0.17 g/L） and tartaric acid （0.18 g/L）, the contents of lactic acid （0.15 g/L） and citric acid （0,15 g/L） were the lowest.
出处
《中国酿造》
CAS
北大核心
2016年第9期154-158,共5页
China Brewing
基金
教育部春晖计划项目(Z2014061)
四川省应用基础项目(2014JY0045)
四川省科技支撑计划(2016FZ0023)
四川省教育厅重点项目(14ZA0110
14Z0107)
