摘要
目的探讨肝肠钙粘连蛋白(liver-intestine cadherin,CDH-17,LI-cadherin)和半乳糖苷凝集素-3(galectin-3)在食管癌组织中的表达及其临床意义。方法采用组织芯片技术和免疫组化SP法检测68例食管鳞癌组织和5例非癌食管黏膜组织中LI-cadherin和galectin-3的表达,并分析其与食管癌临床病理特征的关系。结果 LI-cadherin和galectin-3在食管癌中的阳性表达率分别为70.59%和73.53%,而在非癌食管黏膜组织中LI-cadherin不表达。LI-cadherin表达与浸润深度、淋巴结转移和TNM分期相关(P<0.05),而与分化程度无关(P>0.05)。galectin-3表达与分化程度、浸润深度和淋巴结转移相关(P<0.05),而与TNM分期无关(P>0.05)。LI-cadherin与galectin-3蛋白的表达呈显著正相关(r=0.417,P=0.001)。结论 LI-cadherin在食管癌组织中的高表达与浸润深度、淋巴结转移和TNM分期有关,可能作为一种新的判断转移和预后的标志物,有望成为食管癌治疗的新靶点。
Objective To investigate the expressions and significance of liver-intestine cadherin （LI-cadherin, CDH-17） and galectin-3 in esophageal cancer. Methods Tissue microarray and immunohistochemistry SP method were used to detect the expressions of LI-cadherin and galectin-3 in 68 cases of esophageal cancer tissue and 5 non-cancerous esophageal mucosa. Results There was no staining for LI-cadherin in non-cancerous esophageal mucosa. Positive ex- pressions of LI-cadherin and galeetin-3 were 70.59% and 73.53% in the esophageal cancer, respectively. The positive expression of LI-cadherin was correlated to the depth of invasion, lymphatic metastases and TNM stage of the esophageal cancer （P 〈 0.05 ）, not correlated to the tumor differentiation （P 〉 0.05 ）. The positive expression of galectin-3 was correlated to the tumor differentiation, depth of invasion and lymphatic metastases of the esophageal cancer （P 〈 0.05 ） , but not correlated to TNM stage （P 〉 0.05）. The expressions of LI-cadherin and galectin-3 were positively correlated （ r = 0. 417,P = 0. 001 ）. Conclusion High expression of LI-eadherin is associated with the the depth of invasion, lymphatic metastases and TNM stage of the esophageal cancer. It may be a new marker for metastasis and prognosis of patients with esophageal cancer.
出处
《胃肠病学和肝病学杂志》
CAS
2016年第9期1026-1029,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
2014年大连市医学卫生科学研究计划项目(100)
