摘要
依据GB/T 15000标准样品工作导则,开展了β-烟碱国家标准样品研制工作。采用溶剂提取、萃取、柱色谱、水蒸汽蒸馏等方法制备β-烟碱样品,通过UV,IR,MS和NMR等方法对β-烟碱样品进行结构鉴定,并进行均匀性、稳定性检验。采用8家实验室进行联合定值,对定值结果进行数据分析。β-烟碱标准样品的均匀性和稳定性良好,定值结果为99.12%,置信度95%的扩展不确定度为0.64%。所制得的β-烟碱样品满足标准样品工作导则的要求,可用于有关烟草产品中β-烟碱的检测,以及相关产品的质量控制。
According to the directives for the work of reference materials GB/T 15000,nicotine certified reference material was prepared.Nicotine was prepared by extraction,column chromatography and steam distillation.Its chemical structure was identified by UV，IR，MS and NMR method.Its homogeneity and stability were systematically checked.A cooperative certification was conducted by 8 laboratories，and the results of certification for nicotine were analyzed.The homogeneity and stability of the nicotine reference material were good,and the certified value was 99.12% with expanded uncertainty of 0.64% in confidence coefficient of 95%.The reference material of nicotine conforms to directives for the work of reference materials,and it could be used for method validation and quality control of regarding products.
出处
《分析测试学报》
CAS
CSCD
北大核心
2016年第9期1203-1208,共6页
Journal of Instrumental Analysis
基金
国家质检总局公益性行业科研发展专项(201210209)
关键词
β-烟碱
烟草
标准样品
均匀性
稳定性
定值
nicotine
tobacco
certified reference material
homogeneity
stability
certification
