摘要
采用离子阱飞行时间串联质谱（LCMS-IT-TOF）技术对葡萄酒、果汁等饮料中多组分添加剂和禁用染料进行快速筛查和确证。构建了合成色素、甜味剂、禁用染料等41种化合物的精确分子质量数和多级质谱碎片离子数据库。样品经甲醇一水（体积比1：1）稀释后，以C18色谱柱（150mm3．×4．6mm，5μm）分离，甲醇和2mmol／L乙酸铵为流动相梯度洗脱。筛查检出限为0．005～1．000mg／kg。在3个添加浓度下平均回收率41．2％～114．1％，相对标准偏差（RSD）为4．3％～16．4％。利用精确质量数和数据库中质谱图的检索匹配度实现快速筛查；结合保留时间、同位素丰度和多级特征碎片离子对目标化合物进行确证。该方法具有简便快速、准确、灵敏度高等优点，同时可实现无标准物质的情况下，较短时间内完成对食品中可能存在的添加剂和非法添物进行筛查检测，缩短检测时间，提高检测效率。
A method was developed screening, confirming and quantifying multiple additives and banned dyes in wine, juice and other beverages by liquid chromatography-ion trap-time of flight tandem mass spectrometry （LC-MS-IT-TOF）. The accurate and muhiple-stage mass spectrometry database of 41compounds was established. The analytes in samples were diluted with water-methanol （v/v）. In the chromatographic analysis, target compounds were separated on a CIs column （150 mm×4.6mm, 5μm） witb the gradient elution using the mobile phases of methanol and 2 mM ammonium acetate. The re- sults showed that the limits of detection were 0.005-1.0 mg/kg. The method validation was carried out at three spiking levels, and the recoveries were 41.2 % -114.1%, with the relative standard de- viations （RSDs） of 4.3-16.4 %. The screening of analytes was performed by precision mass matching and library searching. The re- tention time, isotopic abundance and multiple-stage ion mass spectral was employed in this confirmation. This method is simple, fast, credible and highly sensitive and can be applied to screening both multiple and illegal additives in foods without reference standards, reducing the analytical time and improving detection efficiency.
出处
《食品与机械》
CSCD
北大核心
2016年第8期42-48,62,共8页
Food and Machinery
基金
"十二五"国家科技支撑计划课题资助(编号:2012BAK08B01)
粮油深加工与品质控制湖南省2011协同创新项目资助
关键词
食品
添加剂
非法添加物
筛查
高分辨质谱
foods
additives
illegal additives
screening
high resolution mass spectrometry
