摘要
以金属含量、氨基酸组成、差异表达蛋白以及抗氧化酶活性为指标对江山白菇F_(21)品系菇根的生化组成进行了分析。结果表明:与菇身相比,菇根中粗脂肪、铁、磷以及必需氨基酸中的亮氨酸和赖氨酸含量较低,而灰分、砷、钙和镁元素含量较高;通过菇根与菇身的比较蛋白质组学分析,共鉴定出9种差异表达蛋白,涉及双组分信号转导系统、乙酰辅酶A代谢、蛋白质合成、乙醛酸及二羧酸代谢、氮代谢多个生物学过程,同时菇根中的菇毒素含量显著低于菇身;货架期菇根中的抗氧化酶活性较低。
The biochemical properties of fruiting roots of Flammulina velutipes strain Jiangshanbai F21 were evaluated through analysis of metal contents, amino acid composition, differentially expressed proteins and antioxidant enzyme activities in this study. The results showed that fruiting roots contained less crude fats, iron, phosphorus, the essential amino acids leucine and lysine, but contained more ash content, arsenic, calcium and magnesium when compared with fruiting bodies. Using comparative proteomics, 9 differentially expressed proteins were identified which participated in multiple biological processes including two-component signal transduction system, acetyl-CoA metabolism, protein synthesis, glyoxylate and dicarboxylate metabolism, and nitrogen metabolism. Meanwhile, the content of TEER-decreasing protein in fruiting roots was significantly lower than in fruiting bodies. Furthermore, antioxidant enzyme activities in fruiting roots were lower than those in fruiting bodies within the shelf-life period.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第17期90-95,共6页
Food Science
基金
2014年杭州市科技计划项目(20140432B02)
浙江省社科联科普及立项课题(14ZC21)
杭州师范大学本科生创新能力提升工程项目(CX2015098)
关键词
江山白菇F21品系
菇根
氨基酸组成
比较蛋白质组学
抗氧化酶活性
Flammulina velutipes strain Jiangshanbai F21
fruiting root
amino acid composition
comparative proteomics
antioxidant enzyme activities
