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苹果细胞分裂素O–糖基转移酶基因MdZOG1的分离与功能鉴定 被引量：4

Molecular Cloning and Functional Characterization of a Cytokinin O-glycosyltransferase Gene MdZOG1 in Apple
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摘要 以‘嘎拉’苹果为材料,采用同源克隆和PCR技术分离了苹果细胞分裂素O–糖基转移酶基因MdZOG1。MdZOG1的开放阅读框(ORF)长度为762bp,编码含有253个氨基酸的蛋白。系统进化树分析显示,MdZOG1与PbZOG1亲缘关系最近。基因表达分析显示MdZOG1主要在苹果根和茎中表达,在花和果实中的表达量较低。通过农杆菌介导的遗传转化获得转MdZOG1拟南芥和烟草。干旱处理试验结果显示:超量表达MdZOG1显著提高拟南芥和烟草植株的抗旱能力,表明苹果细胞分裂素O–糖基转移酶在植物抗旱胁迫中发挥重要作用。 The cytokinin O-glycosyltransferase gene MdZOG1 was cloned by combination of the homologous clone and PCR technology from apple（Malus×domestica＇Gala＇）.The Open Reading Frame（ORF） of MdZOG1 contained 762 bp,encoding a protein of 253 amino acid residues.Phylogenetic analysis showed that MdZOG1 had the highest evolutionary relationship with PbZOG1.The transcriptional analysis results showed that expression of MdZOG1 was mainly in root and stem,the expression of MdZOG1 in flower and fruit tissues was lower than other tissues.Transgenic Arabidopsis and tobacco were generated by Agrobacterium-msdiated genetic transformation.Ectopic expression MdZOG1 in Arabidopsis and tobacco significantly enhanced resistance to water stress,indicating that cytokinin O-glycosyltransferase played an important role in plant drought stress.
作者 安建平 宋来庆 赵玲玲 由春香 王小非 郝玉金
机构地区 山东农业大学园艺科学与工程学院 烟台市农业科学研究院
出处 《园艺学报》 CAS CSCD 北大核心 2016年第8期1429-1436,共8页 Acta Horticulturae Sinica
基金 国家自然科学基金项目(31430074) 山东省农业良种工程和产业体系岗位专家项目(SDAIT-03-022-03)
关键词 苹果 细胞分裂素O–糖基转移酶 MdZOG1 抗旱胁迫 apple cytokinin O-glycosyltransferase MdZOG1 drought stress
分类号 S661.1 [农业科学—果树学]
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参考文献22

  • 1Chong J, Baltz R, Schmitt C, Beffa R, Fritig B, Saindrenan P. 2002. Down-regulation of a pathogen-responsive tobacco UDP-glucose: phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance. Plant Cell, 14(5): 1093-1107.
  • 2Clough S J, Bent A F. 1998. Floral dip.. a simplified method for Agrobacterium-mediated transformation of.4rabidopsis thaliana. The Plant Journal, 16 (6): 735 - 743.
  • 3Dixon S C, Martin R C, Mok M C, Shaw G, Mok D W. 1989. Zeatin glycosylation enzymes in phaseolus: isolation of O-glucosyltransferase from Phaseolus lunatus and comparison to O-xylosyltransferase from Phaseolus vulgaris. Plant Physiol, 90 (4): 1316 - 1321.
  • 4Fraissinet-Tachet L, Baltz R, Chong J, Kauffmann S, Fritig B, Saindrenan P. 1998. Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyltransferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid. FEBS Letters, 437 (3):319 -323.
  • 5Hou B K, Lim E K, Higgins G S, Bowles D J. 2004. N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana. Journal of Biological Chemistry, 279 (46): 47822 - 47832.
  • 6Jameson P E. 1994. Cytokinin metabolism and compartmentation. Cytokinins: chemistry, activity and function. Boca Raton, FL: CRC Press: 113 - 128.
  • 7Martin R C, Mok M C, Habben J E, Mok D W S. 2001. A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin. Proceedings of the National Academy of Sciences, 98 (10).. 5922 - 5926.
  • 8Martin R C, Mok M C, Mok D W. 1999. A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris. Plant Physiol, 120 (2): 553 - 558.
  • 9Matros A, Mock H P. 2004. Ecotopic expression ofa UDP-glueose: phenylpropanold glucosyltransferase leads to increased resistance oftransgenic tobacco plants against infection with potato virus Y. Plant and Cell Physiology, 45 (9): 1185 - 1193.
  • 10Mok D W S, Mok M C. 1994. Cytokinins, chemistry, activity and function. Boca Raton, FL: CRC Press.

二级参考文献95

  • 1Arend J,Warzecha H, Hefner T, Stockigt J (2001). Utilizing genetically engineered bacteria to produce plant-specific glucosides. Biotechnol Bioeng, 76:126-131.
  • 2Bowles D, Isayenkova J, Lim EK, Poppenberger B (2005). Glycosyltransferases: managers of small molecules. Curr Opin Plant Biol, 8:254-263.
  • 3Chong J, Baltz R, Schmitt C, Beffa R, Fritig B, Saindrenan P (2002). Downregulation of a pathogen-responsive tobacco UDPGlc: phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance. Plant Cell, 14:1093-1107.
  • 4Creelman RA, Mullet JE (1997). Biosynthesis and action of jasmonates in plants. Plant Mol Biol, 48:355-381.
  • 5Fraissinet-Tachet L, Baltz R, Chong J, Kauffmann S, Fritig B, Saindrenan P (I998). Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyltransferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid. FEBS Lett, 437:319-323.
  • 6Frydman A, Weisshaus O, Bar-Peled M, Huhman DV, Sumner LW,Marin FR, Lewinsohn E, Fluhr R, Gressel J, Eyal Y (2004). Citrus fruit bitter flavors: isolation and functional characterization of the gene Cml,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus. Plant J, 40:88-100.
  • 7Fujioka S, Yokota T (2003). Biosynthesis and metabolism of brassinosteroids. Plant Biol, 54:137-164.
  • 8Hoffmeister D,Wilkinson B, Foster G, Sidebottom PJ, lchinose K, Bechthold A (2002). Engineered urdamycin glycosyltransferases are broadened and altered in substrate specificity. Chem Biol, 9:287-295.
  • 9Holton TA, Cornish EC (1995). Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell, 7:1071-1083.
  • 10Horvath DM, Chua NH (1996). Identification of an immediate-early salicylic acidinducible tobacco gene and characterization of induction by other compounds. Plant Mol Biol, 31: 1061-1072.

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园艺学报
2016年 第8期

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