摘要
为探索构建禾草内生真菌不感染试验种群的方法,选用醉马草为材料,以150W和300W微波功率分别处理醉马草种子30、40、50和60s,研究不同微波处理强度下内生真菌的致死情况,同时考察微波对醉马草发芽活力的影响。结果表明,150W和300W处理40s以上,醉马草带菌率分别显著降到19.38%、5.22%以下,300W处理60s,醉马草内生真菌可完全致死;150W处理30、40s和300W处理30s后,醉马草的发芽率、发芽势、发芽指数和活力指数与对照(未经微波处理,CK)相比均无显著差异,其他处理下上述指标随处理强度的增加出现不同幅度的下降;150W或300W处理30s和40s的醉马草芽长、根长和生物量与CK无显著差异,其他处理下各指标均受到不同程度的抑制。因此,150W处理40s为剔除醉马草种传内生真菌的最佳方法。本研究为探索无毒醉马草作为饲料及优良牧草的选育提供了技术支持。
In order to explore and construct the method for endophyte-free populations of Achnatherum inebrians, selecting A. inebrians as the test material and treating its seeds with the microwave power of 150W and 300W for 30,40, 50 and 60s to study the lethal situation of endophyte at different microwave treatment intensity and investigate the effect of microwave to A. inebrians germination vigor. The results indicated that microwave treatment was effective in killing the endophyte in the seeds. While the endophyte were treated 150W or 300W for 40s,the contamination rate significantly dropped below 19.38% and 5.22% ,respectively. After 300W for 60s treatment, seedlings were all uninfected. There was no significant difference between CK and subjects＇ germination rate, germination potential, germination index and vital index after 150W for 30s and 40s or 300 W for 30s. At the same time, the hypocotyls length, radical length and seeding fresh weight were also suppressed after microwave processing, but 150 W or 300 W for 30s and 40s had no significant effect compared with the control. The result showed that the solution of 150 W treated seeds in 40s was the best way to eliminate fungal endophyte of A. inebrians. This study may provide technical support on exploring A. inebrians as animal feed and quality forage.
出处
《核农学报》
CAS
CSCD
北大核心
2016年第9期1792-1797,共6页
Journal of Nuclear Agricultural Sciences
基金
高等学校博士学科点专项科研基金(20126504110001)
新疆农业大学草业科学国家重点学科博士研究生创新基金研究项目(XJCYB-2011-01)
新疆维吾尔自治区研究生教育创新计划科研创新项目(XJGRI2015092)
关键词
内生真菌
微波处理
醉马草
endophyte, microwave treatment, Achnatherum inebrians
