摘要
目的:探讨川芎嗪对模拟失重大鼠颈动脉平滑肌收缩蛋白Ca^(2+)敏感性的影响及机制。方法:21只雌性SD大鼠分为对照组(CON)、模拟失重组(TS)、模拟失重+川芎嗪灌饲组(LTZ)(n=7)。4周后测定大鼠颈动脉收缩性、收缩蛋白Ca^(2+)敏感性及肌球蛋白轻链激酶抑制剂ML-7对以上指标的影响。结果:TS组苯肾上腺素(PHE)收缩力比CON组高25.14%(P<0.01),LTZ组较TS组低13.46%(P<0.01),较CON组高8.30%;ML-7可使CON、TS、LTZ组PHE收缩力分别降低8.84%、16.24%(P<0.01)和3.40%;TS组KCl收缩力比CON组高40.46%(P<0.01),LTZ组较TS组低18.80%,较CON组高14.05%;ML-7可使CON、TS、LTZ组KCl收缩力分别降低21.97%(P<0.05)、21.88%(P<0.01)和10.84%;TS组pD_2[Ca^(2+)]比CON组高10.03%(P<0.01),LTZ组较TS组低7.01%(P<0.01),较CON组高2.32%;ML-7可使CON、TS、LTZ组pD_2[Ca^(2+)]分别降低2.42%、7.43%(P<0.01)和2.51%。结论:模拟失重大鼠颈动脉收缩增强可能是由动脉平滑肌收缩蛋白Ca^(2+)敏感性增强所导致的。川芎嗪可改善模拟失重大鼠颈动脉的收缩功能,其机制可能与抑制血管平滑肌肌球蛋白轻链激酶继而降低Ca^(2+)敏感性有关。
Objective： To study the effects of Ligustrazine on the Ca^2＋ sensitivity in carotid artery smooth muscle of simulated weightlessness rats. Methods： Twenty-one female SD rats were divided into control group （CON）, simulated weightlessness group （TS）, simulated weightlessness plus Ligustrazine administration group （LTZ）（ n = 7）. After 4 weeks, the contractile property and the Ca^2＋ sensitivity in carotid artery were measured. Furthermore, the effects of ML-7（ a myosin light chain kinase specific inhibitor） on above mentioned parameters were recorded. Results： Compared with CON group, the contractile force induced by phenylephrine （PHE） in TS group was increased by 25.14% （ P 〈 0.01）. The force in LTZ group decreased by 13.46% （ P 〈 0.01） and increased by 8.30% compared with TS and CON group, respectively. After the artery rings were incubated with ML-7, the force in CON, TS and LTZ group were reduced by 8.84%, 16.24% （ P 〈 0.01 ） and 3.40%, respectively. Compared with CON group, the contractile force induced by KC1 in TS group was increased hy 40.46% （ P 〈 0.01）. The force in LTZ group decreased by 18.80% and increased by 14.05% compared with TS and CON group, respectively. The force in CON, TS and LTZ group were reduced by 21.97 % （ P 〈 0.05）, 21.88 % （ P 〈 0.01 ） and 10.84% by ML-7, respectively. Compared with CON group, the pD2[Ca^2＋ ] in TS group was increased by 10.03% （P 〈 0.01）. The pD2[Ca^2＋] in LTZ group was decreased by 7. 01% （P 〈 0.01 ） and increased by 2.32% compared with TS and CON group, respectively. The pD2[Ca^2＋] in CON, TS and LTZ group was reduced by 2.42%, 7.43% （P 〈 0.01） and 2.51% by ML-7, respectively. Conclusion： The contraction of carotid artery is strengthened in simulated weightlessness rats, it maybe results from the Ca^2＋ sensitivity of contractile proteins in smooth muscle increasing. Ligustrazine can improve the contraction of carotid artery, reducing the Ca^2＋ sensitivity and inhibiting myosin light chain kinase may be involved in its mechanisms.
出处
《中国应用生理学杂志》
CAS
CSCD
2016年第4期361-364,369,共5页
Chinese Journal of Applied Physiology
基金
陕西省自然科学基金项目(2013JM3001)
陕西省教育厅自然科学专项项目(14JK1755)
西北大学校级大学生创新实验项目(2014004)
