摘要
为考察铅污染大米对人体的潜在健康风险,研究建立体外模拟消化/Caco-2细胞模型来测定大米中铅的生物有效性。结果表明：在模拟大米中铅的胃肠消化过程中,生米和熟米在胃消化阶段铅的生物利用率为61.34%~70.59%和39.69%~47.48%,肠消化阶段分别为24.39%~41.79%和13.57%~15.13%,经胃肠消化后籼米中铅的生物利用率均高于粳米,生米经胃、肠消化生物有效性程度均高于蒸煮后的大米。建立并利用成熟Caco-2细胞模型对大米中铅的生物有效性进行测定,使用四乙基铅与无机铅对大米加标,生米的生物有效性为3.32%和7.0%,熟米的生物有效性为2.65%和5.7%。体外模拟消化/Caco-2细胞模型是一种评价大米中铅生物有效性的有效手段。
A method to determine both the bioaccessibility and bioavailability of lead in rice grains using in vitro digestion/Caco-2 cell model was established. The results indicated that the bioaccessibility of lead in uncooked rice and cooked ricein simulated gastric digestion were 61.34%–70.59% and 39.69%–47.48%, as well as 24.39%–41.79% and 13.57%–15.13%in simulated intestinal digestion, respectively. The lead bioaccessibility of indica rice was higher than that of japonica rice,and uncooked rice was higher than cooked rice. A Caco-2 cell model was established to evaluate the bioavailability of leadin rice. The bioavailability of tetraethyl lead （TTEL） and inorganic lead spiked in cooked rice were 3.32% and 7.0%, whilethose in spiked uncooked rice were 2.65% and 5.7%, respectively. In vitro digestion/Caco-2 cell model can be used as aneffective method to evaluate the bioavailability of lead in rice.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第16期199-204,共6页
Food Science
基金
国家自然科学基金面上项目(31471680)
江苏省自然科学基金项目(BK20131006)
江苏省高校优势学科建设工程资助项目(PAPD)
