摘要
目的探讨临床分离耐甲氧西林表皮葡萄球菌(MRSE)SCCmec型别。方法收集临床分离并经全自动微生物鉴定系统准确鉴定的表皮葡萄球菌84株,通过PCR扩增esp和mecA基因准确鉴定MRSE,采用多重PCR对MRSE SCCmec进行分型,分析其分布特点。结果 PCR扩增结果显示,84株临床分离表皮葡萄球菌均可扩增出esp基因,mecA检出率为76.19%(64/84),其中血液、痰液、尿液和伤口分泌物MRSE检出率分别为76.8%、68.8%、100.0%和71.4%。多重PCR扩增结果显示,64株MRSE中,SCCmec单一型别为22株,其中SCCmecⅠ型19株、SCCmecⅢ型3株;SCCmec混合型为42株,其中Ⅰ、Ⅱ混合型2株,Ⅰ、Ⅲ混合型14株,Ⅰ、Ⅱ、Ⅲ混合型12株,Ⅱ、Ⅲ混合型5株,Ⅲ、Ⅳ混合型9株。结论临床分离的MRSE SCCmec型别存在明显的多样性,以混合SCCmec型别为主。
Objective To investigate the SCCmec types of clinically isolated methicillin‐resistant Staphylococcus epidermidis （M RSE） .Methods Eighty‐four strains of clinically isolated Staphylococcus epidermidis identified by the fully automatic microbio‐logical identification system were collected and performed the MRSE identification by PCR for amplifying esp and mecA genes and SCCmec typing .Its distribution characteristics were analyzed .Results Esp gene was amplified in 84 strains and the detection rate of mecA was 76 .19% （64/84） ,in which the MRSE detection rates in blood ,sputum ,urine and wound secretion were 76 .8% , 68 .8% ,100% and 71 .4% respectively .The multiple PCR amplification displayed that among 64 strains of MRSE ,19 strains were SCCmec simple type ,in which 19 strains were SCCmec type Ⅰ and 3 strains were SCCmec type Ⅲ ;42 strains were SCCmec mixed type ,in which 2 strains were SCCmec mixed type Ⅰ and Ⅱ ,14 strains were SCCmec mixed type Ⅰ and Ⅲ ,12 strains were SCCmec mixed type Ⅰ ,Ⅱ and Ⅲ ,5 strains were SCCmec mixed type Ⅱ and Ⅲ ,a strains were and SCCmec mixed type Ⅲ and Ⅳ .Conclu‐sion The SCCmec type in clinically isolated MRSE shows obvious diversity and its majority is SCCmec mixed type .
出处
《国际检验医学杂志》
CAS
2016年第16期2273-2274,2277,共3页
International Journal of Laboratory Medicine
