摘要
目的:对淡黄香茶菜Isodon flavida地下部分正丁醇部位的化学成分及生物活性进行研究。方法:采用硅胶、羟丙基葡聚糖凝胶(Sephadex LH-20),MCI树脂等多种柱色谱对样品进行分离纯化,运用理化性质和现代波谱技术对单体成分进行结构鉴定;同时,运用1,1-二苯基-2-三硝基苯肼(DPPH)法,β-羟高铁血红素形成抑制法等对样品的抗氧化、抗疟活性进行了初步的研究。结果:从淡黄香茶菜地下部分70%丙酮提取物的正丁醇部位分离得到9个化合物,分别鉴定为2,6-二甲氧基苯醌(1),迷迭香酸甲酯(2),2α-羟基齐墩果酸(3),1-甲氧基-α-D-葡萄糖(4),1-甲氧基-β-D-葡萄糖(5),蔗糖(6),腺花香茶菜苷C(7),5α,6β-二羟基胡萝卜苷(8),(2'R)-1-O-甘油基-6-O-α-D-吡喃半乳糖基-β-D-吡喃半乳糖苷(9);生物活性检测结果显示,化合物2对DPPH有一定的清除作用,同时对β-羟高铁血红素形成也表现出抑制作用,半抑制浓度(IC50)分别为(124.2±2.7),(163.9±7.5)mg·L^(-1)。结论:9个化合物均为首次从该植物中分离得到,化合物2显示出一定的抗疟及抗氧化活性,值得进一步深入研究。本研究为淡黄香茶菜的开发利用提供了一定的实验依据。
Objective： To deeply study the major chemical compounds and their bioactivities of the underground part of Isodonflavida. Method： The samples were isolated and purified by using silica gel, Sephadex LH-20, and MCI chromatographic techniques. The structures of the monomer compositions were determined based on modern spectrum technology and physicochemical properties. In addition, the antioxidant and antimalarial activities of the samples were tested by using 1, 1-diphenyl-2-picrylhydrazyl radical 2, 2-diphenyl-1- （2, 4, 6- trinitrophenyl） hydrazyl （DPPH） method and β-hematin formation inhibition method. Result： Nine compounds were obtained from n-butanol fraction of 70% acetone extract of the underground part of I. flavida, and identified as 2, 6-dimethoxybenzoquinone （ 1 ） , methyl rosmarinate （2） , 2α-hydroxyoleanolic acid （3） , 1-methoxy-α-D- glucose （4）, 1-methoxy-β-D-glucose （5）, sucrose （6）, adenanthoside C （7）, 5α, 6β-dihydroxydaucosterol （ 8 ） , and （2＇R） -1 -O-glyceryl-6-O-α-D-galactopyran-osyl-β-D-galactopyranoside （9）. Bioactive studies indicated that compound 2 showed certain clearing effect on DPPH and inhibitory effect on β-hematin formation, with IC50 values of （124.2±2.7）, （163.9±7.5） mg·L-1, respectively. Conclusion： All of the 9 compounds were isolated from the plant for the first time. Compound 2 showed certain antioxidation and antimalaria effects, which deserved further investigation. This study provided certain experimental basis for the development and utilization of Isodon plants.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第14期60-63,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81460532)
云南省百名海外高层次人才引进计划项目(云组通[2011]123号)
关键词
淡黄香茶菜
地下部分
化学成分
抗氧化活性
抗疟活性
antimalarial activity Isodon flavida
underground part
chemical compounds
antioxidant activity
