摘要
目的了解达州市食源性疾病主动监测沙门菌流行情况、菌型分布、耐药情况,为预防控制食源性疾病发生和临床治疗提供科学依据。方法由哨点医院采集临床就医腹泻患者粪便或肛拭子标本,按照GB/4789.4进行分离鉴定和血清分型;采用WHO推荐的改良K-B纸片法,2014年采用MIC法对分离的沙门菌进行12种抗生素药物敏感试验。结果42株沙门菌共来自4个群14种血清型,其中B群最多47.62%;血清型以鼠伤寒沙门菌最多28.57%、其次为布利丹沙门菌19.05%、肠炎沙门菌11.90%。对磺胺73.81%、氨苄西林55.88%、四环素52.38%、链霉素41.18%和萘啶酸30.95%耐药,对环丙沙星、头孢他啶较敏感;有36株菌(85.71%)具有耐药性,耐3种以上抗生素的有24株菌(57.14%)。结论达州市哨点医院监测沙门菌血清型别复杂多样,且各种血清型别都存在不同程度耐药,加强对沙门菌的监测监控,及时发现新的血清型和耐药特性,为有效防治由沙门菌引发的食品安全事故和临床用药提供可靠依据。
Objective To investigate the epidemic features and drug resistance of foodborne salmonella in Dazhou, and provide the scientific evidence for foodborne diseases treatment and prevention. Methods According to the standard of GB/4789.4, the feces or anal swab samples of diarrhea patients were collected to isolate and serotype. The salmonella isolated were tested by the K - B paper and MIC method for 12 kinds of antibiotics sensitive test. Results There were 42 strains from 14 kinds of serotype in 4 groups, which the B group was the main component. In 14 kinds of serotype, salmonella typhimurium was 28.57% , bliss Dan salmonella was 19.05% , and inflammatory bowel disease salmonella was 11.90%. There were 73.81% salmonella sulfanilamide resistance, 55.88% ampicillin resistance, 52.38% tetracycline resistance, 41.18% streptomycin resistance and 30. 95% nalidixic acid resistance. All strains were sensitive to ciprofloxacin and ceftazidime. There were 36 strains （85.71%） with drug resistance, 24 strains of bacteria （57.14%） with 3 or more kinds of antibiotic resistance. Conclusion The serotype of salmonella was complex in sentinel hospital of Dazhou. The drug resistance was existed in the various serotypes with different level. It should be strengthened that included the monitoring of salmonella on new serotype and drug resistance features, to provide reliable evidence for food safety and clinical treatment of salmonella.
出处
《预防医学情报杂志》
CAS
2016年第8期781-785,共5页
Journal of Preventive Medicine Information
基金
四川省医学会科研课题计划(编号S15070)
关键词
临床分离株
沙门菌
耐药性
clinical isolates
salmonella
drug resistance
