摘要
目的:运用基于8位点的PCR-荧光探针法检测人Y染色体AZF区微缺失情况,分析其结果的可靠性并探讨其对严重少精或无精症患者的临床意义。方法:随机抽取150例严重少精或无精子症患者的外周血,分别运用PCR-荧光探针法和多重PCR凝胶电泳法进行检测Y染色体微缺失AZFa、AZFb、AZFc和AZFd区共8个位点,统计其缺失情况,并分析两方法检验结果的一致性。结果:Y染色体微缺失总发生率为6.00%,非梗阻性无精子症组为9.26%;严重少精子症组为4.17%;PCR-荧光探针法和PCR-电泳法检测结果完全一致。结论:Y染色体AZF区与精子生成关系密切,严重少精子症或非梗阻性无精子症男性行辅助生殖技术前应筛查其微缺失情况;PCR-荧光探针法检测人Y染色体AZF区微缺失稳定快捷,结果可靠。
Objectives： To validate the reliability of detecting Y chromosome microdeletions by fluorescent quantitative PCR（ FQ- PCR） and to discuss the significance for severe oligospermia or azoospermia. Methods：The Y chromosome microdeletions of 150 blood samples of severe oligospermia and azoospermia patients were detected by FQ- PCR and PCR- gel electrophoresis respectively. The data and the concordance of the two methods were classified and analyzed. Results： 8 STSs in area AZFa,AZFb,AZFc and AZFd were detected. The total rate of Y chromosome microdeletions was 6. 00%. The incidence rate was 4. 17% in severe oligospermia group and 9. 26% in azoospermia group. The result of FQ- PCR method was as the same as the PCR- gel electrophoresis. Conclusion：Y chromosome microdeletion has close relationship with male spermatogenesis dysfunction,which has a great significance for detecting Y chromosome in severe oligospermia or azoospermia patients. The method FQ- PCR has advantages of more automatic,stable,rapid and reliable.
出处
《中国性科学》
2016年第6期123-126,共4页
Chinese Journal of Human Sexuality
关键词
荧光定量PCR
Y染色体微缺失
男性不育
Fluorescent quantitative PCR
Y chromosome microdeletions
Male infertility
