摘要
为探讨不同生长阶段团头鲂的赖氨酸需要量,以不同生长阶段的团头鲂[规格Ⅰ:(52.49±0.18)g,规格Ⅱ:(101.85±1.8)g]为研究对象,配制赖氨酸含量为1.29%、1.71%、2.09%、2.48%、2.88%和3.27%的等氮等能饲料进行10周养殖实验。结果表明:与对照组相比,(1)2.88%和3.27%组显著提高了规格Ⅰ的增重率、特定生长率和蛋白质效率,显著降低了饵料系数和摄食量;2.48%组显著提高了规格Ⅱ的特定生长率,显著降低了饵料系数,3.27%组提高了规格Ⅱ的特定生长率和摄食量;(2)2.88%和3.27%组分别显著提高了规格Ⅰ和规格Ⅱ的鱼体粗脂肪含量;(3)2.48%组显著降低了规格Ⅰ和规格Ⅱ的血清谷丙转氨酶活性,显著提高了规格Ⅰ的尿素含量;3.27%组显著提高了规格Ⅱ的血清白蛋白、总蛋白和球蛋白含量;表明适宜水平赖氨酸可能对鱼体肝脏具有保护作用,能够提高其代谢及免疫力;(4)2.48%、2.88%和3.27%组均显著提高了规格Ⅰ和规格Ⅱ的血清赖氨酸含量,显著降低了规格Ⅰ的血清精氨酸和总必需氨基酸含量。以鱼体特定生长率为评价指标,经折线模型回归分析得到规格Ⅰ和规格Ⅱ的最适赖氨酸需要量分别为饲料干重的2.07%和2.19%(饲料粗蛋白含量的6.27%和6.63%)。
A 10-week feeding trial was conducted to investigate the dietary lysine requirement of blunt snout bream（ Megalobrama amblycephala） with initial body weight of 52. 49 ± 0. 18 g（ size Ⅰ） and 101. 85 ± 1. 85g（ size Ⅱ）. Six isonitrogenous and isoenergetic semi-purified diets were formulated that containing graded lysine levels（ 1. 29%,1. 71%,2. 09%,2. 48%,2. 88% and 3. 27% of dry weight）. The results showed thatweight gain rate（ WGR）,specific growth rate（ SGR） and protein efficiency ratio（ PER） in fish of sizeⅠ fed with diets containing 2. 88% and 3. 27% lysine were significantly higher than that of the control group,while the reverse pattern was observed in feed conversion ratio（ FCR） and feed intake（ FI）. The 2. 48%lysine diet significantly increased SGR and decreased FCR,meanwhile,3. 27% lysine diet significantly increased SGR and FI in fish of size Ⅱ. The diet with 2. 88% and 3. 27% lysine significantly increased body fat content in fish of size Ⅰ and size Ⅱ,respectively. Significantly decreased ALT in plasma of size Ⅰ and Ⅱand increased Urea of size Ⅰ were observed fed with 2. 48% lysine diet,while increased ALB,TP and GLB in plasma content of size Ⅱ fed with 3. 27% lysine diet,which indicate that proper lysine in diet might protect the liver and enhance the metabolism and immunity ability compare with the control group. The2. 48%,2. 88% and 3. 27% lysine diet significantly increased the plasma lysine content of size Ⅰ and Ⅱ,and decreased the plasma arginine and total essential amino acids content of size Ⅰ. Based on the broken-line regression analysis of SGR,the optimal dietary lysine requirements of size Ⅰ and Ⅱ were estimated to be2. 07% and 2. 19%（ 6. 27% and 6. 63% of dietary protein）,respectively.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2016年第3期396-405,共10页
Journal of Shanghai Ocean University
基金
公益性行业(农业)科研专项经费(201003020)
现代农业产业技术体系建设专项资金项目(CARS-46)
关键词
团头鲂
不同生长阶段
赖氨酸
需要量
生长
Megalobrama amblycephala
different growth stage
lysine
requirement
growth performance
