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肽基脯氨酰顺反异构酶B对胰岛细胞中胰岛素表达的影响

Effect of peptidly-prolyl isomerase B on insulin expression in islet β cells
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摘要 目的探讨肽基脯氨酰顺反异构酶B(PPIB)对大鼠胰岛β细胞中胰岛素合成的影响。方法用不同浓度环孢素A 0、0.1、0.5、1、2.5、5、7.5、10、12.5和15μmol/L处理INS1细胞,采用MTT法检测INS1细胞活力,Western blot法检测INS1细胞中PPIB和胰岛素表达水平。结果环孢素A呈时间和剂量依赖性地抑制INS1细胞活力,降低PPIB和胰岛素含量(P<0.05或P<0.01)。结论环孢素A可能通过下调PPIB表达抑制胰岛细胞中的胰岛素合成。 Objective To investigate the effect of peptidly-prolyl isomerase B（PPIB）on the synthesis of insulin in rat isletβcells.Methods The INS1 cells were treated with different concentrations of cyclosporin A 0,0.1,0.5,1,2.5,5,7.5,10,12.5 and 15 μmol/L.The cell viability and insulin expression were detected by MTT assay and Western blot,respectively.Results Cyclosporin A inhibited cell viability in dose-and time-dependent manners and decreased the expressions of PPIB and insulin（P〈0.05 or P〈0.01）.Conclusion Cyclosporin A can reduce insulin production in rat islet β cells probably by down-regulating PPIB expression.
作者 朱丹 韦晓 曹萌 茅晓东 朱辉 浮迎迎 刘超 蔡可英
机构地区 徐州医科大学 南京中医药大学附属中西医结合医院内分泌代谢病科 徐州医科大学第二附属医院内分泌病科
出处 《江苏医药》 CAS 2016年第10期1108-1110,共3页 Jiangsu Medical Journal
基金 江苏省自然科学基金项目(BK2012887)
关键词 肽基脯氨酰顺反异构酶B 胰岛素 胰岛Β细胞 Peptidly-prolyl isomerase B Insulin Islet β cells
分类号 R335 [医药卫生—人体生理学]
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参考文献11

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二级参考文献11

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江苏医药
2016年 第10期

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