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人FKBP3在哺乳动物细胞系中的转染表达与定位 被引量：1

Expression and localization of transfected human FKBP3 in mammalian cell lines
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摘要 以含cDNA序列的质粒为模板,下游引物带有FLAG标签,PCR扩增,插入pcDNA3.1,成功构建C端带FLAG标签的人野生型肽基脯氨酰顺反异构酶FKBP3的哺乳动物细胞表达载体pcDNA3.1-FKBP3-FLAG。转染HEK 293T细胞,Western blot检测;转染COS-7细胞,荧光显微镜观察。FKBP3在HEK 293T、COS-7细胞中均有表达,且定位在COS-7细胞的细胞质和细胞核,胞质分布较多,为进一步研究FKBP3的相互作用蛋白和功能奠定了一定的基础。 The full length cDNA fragment of FKBP 3 from plasmid was used for PCR amplification with FLAG tag inthe downstream primer. The PCR product was inserted into pcDNA3.1 vector, to construct the mammalian cell expression vector of wild type FKBP3 with FLAG tag in C-terminal pcDNA3.1-FKBP3-FLAG. Its expression was detected in transfected HEK 293T cells with Western blot. The localization of FKBP3 protein in transfected COS-7 cells was observed by immunofluorescence. The transfected ceils can express FKBP3-FLAG efficiently in the two cell lines, and the FKBP3 protein was found to localize in both cytoplasm and nucleus in COS-7 cell line, with majority in the cytoplasm. The results provide the basis for further studying of the function and interaction partners of FKBP3 protein.
作者 龚芮 潘林鑫 张姗靖 耿慧武 刘晓颖 范礼斌
机构地区 安徽医科大学生命科学院生物学教研室
出处 《安徽医科大学学报》 CAS 北大核心 2013年第5期558-561,共4页 Acta Universitatis Medicinalis Anhui
基金 安徽省教育厅自然科学重点科研项目(编号:KJ2010A187) 安徽省自然科学基金项目(编号:11040606M170 11040606M164)
关键词 FKBP3 基因表达 细胞定位 免疫荧光 FKBP3 gene expression cellular localization immunofluorescence
分类号 R341 [医药卫生—基础医学] R394.2 [医药卫生—医学遗传学]
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参考文献14

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共引文献1

同被引文献6

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  • 6潘林鑫,李新颖,徐南,李克娟,乔正,耿慧武,刘晓颖,范礼斌.人RB1及其突变体的表达与定位[J].安徽医科大学学报,2013,48(8):853-858. 被引量：6

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安徽医科大学学报
2013年 第5期

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