摘要
建立超高效液相色谱法,以0.01%甲酸乙腈-0.01%甲酸水为流动相进行梯度洗脱,同时测定西洋参样品中6种皂苷-人参皂苷Rg_1,Re,Rb_1,Rc,Ro,Rd的质量分数;应用SPSS 21.0及SIMCA-P软件,对西洋参药材与饮片、不同生长年限西洋参样品数据进行方差分析、主成分分析和偏最小二乘判别分析。在所建立的色谱条件下,6种人参皂苷能够达到良好分离,线性关系、稳定性、精密度、重复性、加样回收试验均符合中药质量分析要求;所测57份西洋参样品中人参皂苷Rg_1,Re,Rb_1的总质量分数都符合2015年版《中国药典》的规定;方差分析结果显示,西洋参药材和饮片中6种人参皂苷总质量分数有显著性差异,主成分分析可以实现药材与饮片的区分;二至四年产样品中人参皂苷质量分数随生长年限的增长而增长,偏最小二乘判别分析可用于区分二至四年生西洋参样品。研究结果表明,该实验建立的超高效液相色谱法快速、准确,可用于西洋参样品中人参皂苷类成分的含量测定;多元数据分析适合于西洋参的质量分析。
An ultra performance liquid chromatography（ UPLC） method combined with multivariate data analysis was developed to evaluate the quality of American ginseng by simultaneously determining the concentrations of six ginsenosides（ Rg_1,Re,Rb_1,Rc,Ro and Rd） in the samples. For UPLC,acetonitrile with 0. 01% formic acid and water with 0. 01% formic acid were used as the mobile phase with gradient elution. Under the established chromatographic conditions,the six ginsenosides could be well separated and the results of linearity,stability,precision,repeatability,and recovery rate all reached the requirement of quantification analysis,respectively. The total contents of Rg_1,Re,and Rb_1 in 57 samples all reached the requirement of the 2015 edition of Chinese Pharmacopoeia.At the same time,the experimental data were analyzed by principle component analysis（ PCA） and partial least squares discriminant analysis（ PLS-DA）. The crude drugs and the decoction pieces can be discriminated by a PCA method and the samples with different age can be distinguished by a PLS-DA method.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第9期1678-1684,共7页
China Journal of Chinese Materia Medica
基金
中医药行业科研专项项目(201407003)
