摘要
应用超高效液相色谱-四极杆飞行时间串联质谱联用技术（UPLC-Q-TOF-MS）对川西吊石苣苔的化学成分进行定性研究。采用Waters ACQUITY UPLC-BEH-C18S色谱柱（2.1 mm×100 mm,1.7μm）,以0.1%甲酸水-乙腈为流动相梯度洗脱,检测波长210-400 nm,ESI负离子模式进行检测,根据精确分析量及碎片信息,结合数据库匹配进行结构鉴定。结果从川西吊石苣苔中共鉴定出57个成分,其中42个苯乙醇苷类成分,5个苯甲醇苷类成分,6个黄酮类成分,4个其他类型化合物,其中有43个化合物为苦苣苔科中首次发现,并初步推测了1个新苯甲醇苷类化合物。该研究首次对川西吊石苣苔化学成分进行整体研究,为该植物的深入研究奠定基础。
The Ultra-high Performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry（ UPLC-Q-TOFMS） was applied to analyze the chemical components in Lysinotus wilsonii. A Waters ACQUITY UPLC-BEH-C18 S column（ 2. 1mm × 100 mm,1. 7 μm） was used with a gradient elution of acetonitrile-water containing 0. 1 % formic acid. The mass spectrometry equipped with ionization source was used and the data was collected in negative ion mode. Results showed that 57 components were identified as 42 phenylethanoid glycosides,5 benzyl alcohol glycosides,6 flavonoids and 4 other components. Among them,43 compounds were firstly identified in Gensneriaceae and one benzyl alcohol glycoside may be a new compound. We have quite completely identified the components in L. wilsonii for the first time,which may lay the foundation for further study and utilization of the medicinal plant.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第9期1658-1669,共12页
China Journal of Chinese Materia Medica
基金
中医药行业科研专项(201407003)
关键词
液质联用
川西吊石苣苔
化学成分
UPLC-Q-TOF-MS
Lysinotus wilsonii
chemical constituents
