摘要
目的:利用UPLC-ESI-HRMSn技术对三草保肝汤中化学成分进行分析。方法:采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以甲醇(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱(0~2 min,5%A;2~20 min,5%~12%A;20~35 min,12%~40%A;35~38 min,40%A;38~48 min,40%~80%A;48~50 min,80%A),流速0.3 m L·min-1,柱温30℃,进样量10μL;采用电喷雾离子源,在正、负离子模式下采集数据。运用Xcalibur 3.0软件,根据化合物的相对保留时间、准分子离子峰和碎片离子峰等信息,结合人类代谢组数据库(HMDB),参考相关文献以及对照品比对,鉴定三草保肝汤的主要化学成分。结果:从三草保肝汤中鉴定出了40个化学成分,包括酚酸类16个、黄酮类19个、蒽醌类2个、三萜类1个、甾醇类1个、单萜类1个,其中有6个化学成分(α-蒎烯、丹参素、表没食子儿茶素、龙胆酸、柚皮素、大黄素)首次从该复方中分离得到。结论:UPLC-ESI-HRMSn技术能建立快速、准确分析三草保肝汤中化学成分的方法,对三草保肝汤化学成分进行了较为全面的定性分析,为该复方的药效物质基础研究及质量控制奠定了基础。
Objective:To analysis the chemical constituents in Sancao Baogan decoction by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-ESI-HRMSn).Method:The separation was performed on an ACQUITY UPLC BEH C18column(2.1 mm×100 mm,1.7μm)by a gradient elution of methanol(A)-0.1%formic acid solution(B)(0-2 min,5%A;2-20 min,5%-12%A;20-35 min,12%-40%A;35-38 min,40%A;38-48 min,40%-80%A;48-50 min,80%A).The flow rate was 0.3 m L·min-1and the column temperature was set at 30℃,the injection volume was 10μL.Electrospray ionization was applied and the data were collected via positive and negative ion modes.By using Xcalibur 3.0 software,the chemical constituents were analyzed based on the relative retention time,excimer ion peak and fragment ion peak of the compounds,as well as comparison with human metabolome database(HMDB),reference substances and literature data.Result:A total of 40 chemical components were identified from Sancao Baogan decoction,including 16 phenolic acids,19 flavonoids,2 anthraquinones,1 triterpenoid,1 sterol,and1 monoterpenoid.Six compounds(dansensu,α-pinene,epigallocatechin,2,5-dihydroxybenzoic acid,naringenin and emodin)were reported for the first time from Sancao Baogan decoction.Conclusion:The established UPLC-ESI-HRMSncan quickly,accurately and comprehensively analyze the chemical constituents of Sancao Baogan decoction,which lays a foundation for the basic research of pharmacodynamic substances and quality control of this formula.
作者
刘香易
曾锐
杨长传
彭靖兰
严亨秀
LIU Xiang-yi;ZENG Rui;YANG Chang-chuan;PENG Jing-lan;YAN Heng-xiu(College of Pharmacy,College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第7期141-147,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省科技厅应用基础研究项目(2018JY0069)
西南民族大学研究生创新性科研项目(XC2018SZ22)
中央高校基本科研业务费专项(2018NZD16).
关键词
三草保肝汤
化学成分
酚酸类
黄酮类
蒽醌类
萜类
甾醇类
Sancao Baogan decoction
chemical composition
phenolic acids
flavonoids
anthraquinones
triterpenoids
sterols
