期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

T0901317通过上调LXRα表达促进人乳腺癌MDA-MB-231细胞凋亡 被引量：2

T0901317 induces apoptosis of human breast cancer MDA-MB-231 cells by up-regulating LXRα expression
暂未订购
导出
摘要 目的:观察肝X受体(LXR)激动剂T0901317对人乳腺癌细胞MDA-MB-231凋亡的作用及其机制。方法:不同浓度(0、10、20、40μmol/L)T0901317处理人乳腺癌MDA-MB-231细胞不同时间(0、12、24、48 h),用Hoechst 33342染色及流式Annexin V-FITC/PI双染法检测细胞凋亡;Western blot进一步检测细胞凋亡相关蛋白Bcl-2、cleaved caspase-3和LXRα的表达,RT-q PCR检测Bcl-2和LXRα的mRNA表达。结果:随着T0901317处理浓度的增加和时间的延长,凋亡现象逐渐明显。进一步通过Western blot检测发现,T0901317可下调Bcl-2蛋白表达,cleaved caspase-3表达增多,而LXRα的表达上调;RT-q PCR检测结果也显示,T0901317可下调Bcl-2 mRNA表达,而上调LXRα表达。结论:T0901317能上调LXRα表达,从而促进MDA-MB-231细胞凋亡。 AIM： To investigate the pro-apoptotic effect of T0901317,an artificial agonist of liver X receptor α（ LXRα）,on human breast cancer MDA-MB-231 cells and its mechanism. METHODS： MDA-MB-231 cells were treated with different concentrations（ 0,10,20 and 40 μmol / L） of T0901317 for different time（ 0,12,24 and 48 h）. The cell apoptosis was determined by Annexin V / propidium iodide staining and Hoechst 33342 staining. The expression of apoptosisrelated proteins,such as Bcl-2,caspase 3 and cleaved caspase-3,and LXRα was determined by Western blot. The mRNA expression of Bcl-2 and LXRα was analyzed by RT-q PCR. RESULTS： T0901317 induced the cell apoptosis in a dose-and time- dependent manner. The expression of cleaved caspase-3 and LXRα was up-regulated,but Bcl-2 was down-regulated by T0901317. The mRNA expression of Bcl-2 was down-regulated,while LXRα was up-regulated by T0901317. CONCLUSION： T0901317 up-regulates LXRα expression and induces the apoptosis of MDA-MB-231 cells.
作者 涂剑 陆凯强 丁维珂 刘晓旺 熊婷 刘伶 严欣 周志刚
机构地区 南华大学药物药理研究所 南华大学第一附属医院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2016年第5期836-840,共5页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.81541163) 湖南省自然科学基金资助项目(No.2015JJ3101) 湖南省教育厅创新平台项目(No.15K111) "湖南省分子靶标新药研究协同创新中心"培育项目(No.2014-405)
关键词 T0901317 肝X受体Α MDA-MB-231细胞 细胞凋亡 T0901317 Liver X receptor α MDA-MB-231 cells Apoptosis
分类号 R730.23 [医药卫生—肿瘤]
  • 相关文献

参考文献18

  • 1El Roz A, Bard JM, Valin S, et al. Macrophage apolipoprotein E and proliferation of MCF-7 breast cancer cells:role of LXR[J]. Anticancer Res, 2013, 33(9):3783-3789.
  • 2Chuu CP. Modulation of liver X receptor signaling as a prevention and therapy for colon cancer[J]. Med Hypo-theses, 2011, 76(5):697-699.
  • 3Yang CM, Lu YL, Chen HY, et al. Lycopene and the LXRα agonist T0901317 synergistically inhibit the proliferation of androgen-independent prostate cancer cells via the PPARγ-LXRα-ABCA1 pathway[J]. J Nutr Biochem, 2012, 23(9):1155-1162.
  • 4Jia Y, Viswakarma N, Reddy JK. Med1 subunit of the mediator complex in nuclear receptor-regulated energy metabolism, liver regeneration, and hepatocarcinogenesis[J]. Gene Expr, 2014, 16(2):63-75.
  • 5Zhang W, Jiang H, Zhang J, et al. Liver X Receptor activation induces apoptosis of melanoma cell through caspase pathway[J]. Cancer Cell Int, 2014, 14(1):1-6.
  • 6Pommier AJ, Alves G, Viennois E, et al. Liver X receptor activation downregulates AKT survival signaling in lipid rafts and induces apoptosis of prostate cancer cells[J]. Oncogene, 2010, 29(18):2712-2723.
  • 7El Roz A, Bard JM, Huvelin JM, et al. LXR agonists and ABCG1-dependent cholesterol efflux in MCF-7 breast cancer cells:relation to proliferation and apoptosis[J]. Anticancer Res, 2012, 32(7):3007-3013.
  • 8EBCTCG(Early Breast Cancer Trialists' Collaborative Group). Effect of radiotherapy after mastectomy and axillary surgery on 10-year recurrence and 20-year breast cancer mortality:meta-analysis of individual patient data for 8135 women in 22 randomised trials[J]. Lancet, 2014, 383(9935):2127-2135.
  • 9陆英,刘相富,刘玲玲,李芳,覃雪玲,林东军.ARHI基因抑制U937白血病细胞株生长并诱导其G_2/M期阻滞及凋亡[J].中国病理生理杂志,2015,31(11):1950-1955. 被引量：3
  • 10Delgado ME, Olsson M, Lincoln FA, et al. Determining the contributions of caspase-2, caspase-8 and effector caspases to intracellular VDVADase activities during apoptosis initiation and execution[J]. Biochim Biophys Acta, 2013, 1833(10):2279-2292.

二级参考文献26

  • 1Dohner H, Stilgenbauer S, Benner A, et al. Genomic aberrations and survival in chronic lymphocytic leukemia[J]. New Eng J Med, 2000, 343(26):1910-1916.
  • 2Wenming C, Jiazhi Z, Shuzhen T, et al. Inactivation of p16 gene in leukemia[J]. Chin Med Sci J, 1999, 14(4):206-210.
  • 3Kawano S, Miller CW, Gombart AF, et al. Loss of P73 gene expression in leukemias/lymphomas due to hypermethylation[J]. Blood, 1999, 94(3):1113-1120.
  • 4Luo RZ, Fang X, Marquez R, et al. ARHI is a Ras-related small G-protein with a novel N-terminal extension tha inhibits growth of ovarian and breast cancer[J]. Oncogene, 2003, 22(19):2897-2909.
  • 5Yu Y, Xu F, Peng H, et al. NOEY2(ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas[J]. Proc Natl Acad Sci U S A, 1999, 96(1):214-219.
  • 6Rosen DG, Wang L, Jain AN, et al. Expression of the tumor suppressor gene ARHI in epithelial ovarian cancer is associated with increased expression of p21WAF1/CIP1 and prolonged progression-free survival[J]. Clin Cancer Res, 2004, 10(19):6559-6566.
  • 7Lu Z, Luo RZ, Peng H, et al. Transcriptional and posttranscriptional down-regulation of the imprinted tumor suppressor gene ARHI (DRAS3) in ovarian cancer[J]. Clin Cancer Res, 2006, 12(8):2404-2413.
  • 8Fujii S, Luo RZ, Yuna J, et al. Reactivation of the silenced and imprinted alleles of ARHI is associated with increased histone H3 acetylation and decreased histone H3 lysine 9 methylation[J]. Hum Mol Genet, 2003, 12(15):1791-1800.
  • 9Feng W, Marquez RT, Lu Z, et al. Imprinted tumor suppressor genes ARHI and PEG3 are the most frequently down-regulated in human ovarian cancers by loss of heterozygosity and promoter methylation[J]. Cancer, 2008, 112(7):1489-1502.
  • 10Hisatomi H, Nagao K, Wakita K, et al. ARHI/NOEY2 inactivation may be important in breast tumor pathogenesis[J]. Oncology, 2002, 62(2):136-140.

共引文献2

同被引文献12

引证文献2

二级引证文献6

中国病理生理杂志
2016年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部