摘要
研究以壳聚糖膜为载体、戊二醛为交联剂,固定化葡萄糖氧化酶的最佳条件。结果表明,采用脱乙酰度为86.40%,分子量为1.288×106的壳聚糖制作壳聚糖膜,与浓度0.025%的戊二醛交联后做为载体,室温状态下,在pH为6.5、酶浓度为0.2mg/ml的酶液中固定12h,固定化酶活力最大。在此条件下,固定化酶活力为186.8U/cm2,相对活力78.69%,活力回收>40%。
Glucose oxidase can be covalently immobilized on glutaradehyde-pretreated chitosan membrane. The chitosen menbrance with degree of deacetylation of 86.44% and with a melocular weight of 1.288×106 Datlon is used as support for enzyme immobilization.The optimum conditions for immobilizing glucose oxidase on glutaradehyde-pretreated chitosan membrane is 0.025% glutaradehyde pH6.5 12hours and 0.2mg/ml of the enzyme concentration at the room temperature. The immobilized enzyme activity is 186.8U/cm2 relative activity is 78.69% and the recovery rate of the enzyme is above 40%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2002年第8期9-12,共4页
Science and Technology of Food Industry
