摘要
目的研究青蒿琥酯干预下表达Foxp3的调节性T细胞在大鼠胶原诱导性关节炎(CIA)脾淋巴细胞及滑膜中表达及意义。方法建立大鼠CIA模型,分为对照组、CIA模型组、CIA模型+不同浓度青蒿琥酯组、CIA模型+甲氨蝶呤(MTX)组、CIA模型+硫酸羟氯喹组、CIA模型+甲基泼尼松龙组。流式检测脾淋巴细胞中Foxp3表达,免疫组化检测Foxp3在大鼠踝关节滑膜细胞中表达。结果 CIA模型+20mg·kg^(-1)·d^(-1)青蒿琥酯组中Foxp3在脾淋巴细胞及滑膜组织中的表达明显高于其他浓度青蒿琥酯组、CIA模型+硫酸羟氯喹组(P<0.05),与CIA模型+甲基泼尼松龙组在脾淋巴细胞表达中有差异(P<0.05),与CIA模型+甲氨蝶呤组无差异(P>0.05)。结论青蒿琥酯可上调T淋巴细胞及滑膜中Foxp3的表达,且与剂量呈正相关,当剂量在20mg·kg^(-1)·d^(-1)时效果最明显。
Objective To investigate the expression of regulatory T cells expressing Foxp3 in rat synovial cells and T lymphocytes of collagen-induced arthritis（CIA） through artesunate intervention and their significance. Methods The CIA model in SD rats was established and divided into the control group, CIA model group, CIA and different concentrations of artesunate groups, CIA and methotrexate（MTX） group, CIA and hydroxychloroquine （HCQ） group, CIA and methylprednisolone （MP） group. The lymphocytes were extracted from spleen. The expression of Foxp3 in different groups of CIA rats were detected by flow cytometry （FCM）. The immunohistochemical method was used to detect Foxp3 expression in rats ankle synovial cells. Results Foxp3 expression in spleen lymphocyte and synovial tissue in CIA and 20 mg· kg-1·d-1 artesunate group was obviously higher than that in other artesunate groups,CIA and hydroxychloroquine group（P〈0.05）, but the expression of Foxp3 in the CIA and spleen lympho- cytes had statistical difference compared with CIA and methylprednisone group and had no statistical difference compared with the CIA and methotrexate group（P〉0.05）. Conclusion Artesunate can up-regulate the expression of Foxp3 in T lymphocytes and synovial tissue,moreover is positively correlated with the dosage. When the dose is 20 mg·kg-1·d-1 , the effect is most significant.
出处
《重庆医学》
CAS
北大核心
2016年第13期1729-1732,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(81160376
81360462)
广西自然科学基金资助项目(2013GXNSFAA019111)
