摘要
为评价不同蛋白酶水解蜂王浆蛋白的效果,本研究以蛋白酶K为试验用酶,对蜂王浆蛋白的最佳水解条件及水解产物稳定性进行了研究。结果显示,蛋白酶K水解蜂王浆的最佳条件为温度55℃,p H 7.5,酶添加量60μg/m L,氯化钙添加量0.01 mol/L,反应时间4 h,水溶性蛋白质提取率为31.03%;蛋白酶K水解蜂王浆蛋白的产物在酸、碱、热条件下能够保持较高的稳定性。蛋白酶K水解蜂王浆的效果介于植物性蛋白酶及动物性蛋白酶之间,可用于生产富钙型的水溶性蜂王浆。
For studying different protease hydrolysis of royal jelly,water-soluble royal jelly was produced by proteinase K which used for biochemical test,and the optimal hydrolysis conditions of proteinase K and stability of enzymatic hydrolysates were studied in this paper. The results showed that the optimal hydrolysis of proteinase K was 55 ℃,p H 7. 5,proteinase K 60 μg / m L,Ca Cl20. 01 mol / L,and time 4 h,protein extraction rate 31. 03 %. The stability of enzymatic hydrolysates was better. The effect of proteinase K hydrolysis of royal jelly was between plant proteinase and animal proteinase. Proteinase K could be used in the production of rich calcium type of water-soluble royal jelly.
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2016年第2期288-293,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
国家现代农业(蜂蜜)产业技术体系建设专项(CARS-45-SYZ15)
重庆市农发资金项目(14428)
重庆市前沿与应用基础研究(cstc2015jcyjA80032)
关键词
蜂王浆
蛋白酶K
水溶性蛋白质提取率
正交试验
royal jelly
proteinase K
extraction rate of water-soluble protein
orthogonal experiment
