摘要
为了探究外源甜菜碱对盐胁迫下黑果枸杞(Lycium ruthenium)种子萌发及抗氧化物酶系活性的影响,对采自于河西走廊黑河中游的黑果枸杞进行种子萌发及幼苗生长试验,测定其发芽率(GP)、发芽势(GV)、发芽指数(GI)、活力指数(VI)、相对盐害率和抗氧化物酶系[超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)]等指标。结果显示,25mmol·L^(-1)的NaCl处理,对黑果枸杞种子的萌发具有促进作用,50~300mmol·L^(-1)的NaCl处理则有抑制作用;黑果枸杞种子萌发的NaCl浓度的临界值是50mmol·L^(-1),极限值是300mmol·L^(-1);随着NaCl浓度的增加,SOD、POD和CAT活性均不同程度地表现为"先上升,后下降"的趋势。在添加外源甜菜碱处理后,各NaCl浓度处理下的SOD、POD和CAT活性均有不同程度的增加。综上表明,甜菜碱能有效减缓盐胁迫对黑果枸杞种子萌发及幼苗生长产生的伤害,提高种子及幼苗的抗盐能力。
In order to investigate the effects of exogenous betaine on seed germination,activities of antioxidase enzymes of Lycium ruthenium under NaCl stress.The L.ruthenium seed germination and seedling growth were studied in middle reaches of Heihe River of Hexi Corridor.Several physiological indexes,such as the germination percentage（GP）,germination vigor（GV）,germination index（GI）,vigor index（VI）,and relative salt damage rate,antioxidant enzymes（SOD,POD and CAT）were measured.The results indicated that,low NaCl concentration（25mmol·L^-1）could promote the seed germination but high NaCl concentration（50-300mmol·L^-1）could inhibit the seed germination.The NaCl concentration threshold for seed germination in L.ruthenium was 50mmol·L^-1,the maximum was 300mmol·L^-1;The SOD,POD and CAT activity showed a trend of first rising and then falling along with the salt concentration increasing.After the treatment by exogenous betaine,SOD,POD and CAT activity increased to some extent,but they had less decrease range under NaCl stress.Exogenous betaine could significantly alleviate the damages to the seeds and seedlings of L.rutheniumunder NaCl stress and promote the salt resistance of the seeds and seedlings.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第4期674-680,共7页
Pratacultural Science
基金
国家自然科学基金--黑河下游荒漠植物黑果枸杞分布格局及盐碱适应性研究(41261013)
甘肃省青年科技基金计划--黑河流域荒漠植被生态化学计量特征及其机制研究(1308RJYA031)
甘肃省自然科学基金金--河西走廊常见栽培中药材耐碱特性的研究(145RJZG191)
关键词
甜菜碱
盐胁迫
种子萌发
抗氧化酶
Lycium ruthenicum
exogenous glycinebetaine
NaCl stress
seed germination
antioxidase
