摘要
目的:建立测定大鼠肺组织中氟康唑的高效液相色谱-紫外吸收检测(HPLC-UV)法,探讨氨溴索对大鼠肺组织中氟康唑药物浓度的影响。方法:将实验大鼠按随机数字表法分为A、B组,每组各10只,A组大鼠首日经尾静脉给予72 mg/kg注射用氟康唑,以后给予1日36 mg/kg;B组大鼠先经胃灌注给予6 mg/kg氨溴索,1日2次,上午给予氨溴索30 min后给予注射用氟康唑。连续给药2 d后,第3日给予氟康唑后2 h对大鼠颈静脉取血后解剖,取肺组织备用。采用HPLC-UV法同时测定最后一次使用氟康唑后2 h大鼠的肺组织浓度。结果:氟康唑线性范围均为2.5~80μg/ml之间,回归方程为X=0.052C-0.027 6,相关系数r=0.999 9。A组大鼠肺组织氟康唑的浓度(28.1±19.6)μg/ml,B组为(26.6±17.7)μg/ml,2组的差异无统计学意义(P<0.05)。结论:氨溴索对肺组织中的氟康唑药物浓度无显著影响,但尚需结合临床实际行进一步的研究。
OBJECTIVE： To establish a HPLC-UC method of fluconazole and explore effects of ambroxol on concentration of fluconazole in rat lung tissue. METHODS： Rats were divided into group A and group B via the random number table,with 10 cases in each. And group A were treated with 72 mg / kg fluconazole injection by caudal vein in the first day,followed by 36 mg / kg for a day during the latter treatment. Group B received 6 mg / kg ambroxol by abomasum infusion,twice a day,and after the administration of ambroxol for 30 min in the morning,fluconazole injection was given. After administration of 2 d,2 h after administration of fluconazole in the 3^（rd） day,the blood was drawn from jugular vein of rats and then anatomy was conducted,lung tissue of rats were taken. HPLC-UV method was adopted to determine the concentration of rat lung tissue 2 hours after injecting fluconazole in the last administration.RESULTS： The calibration curve was linear with the logarithm of concentration range of 2. 5 ~ 80 μg / ml of fluconazole,he linear equation was X = 0. 052C- 0. 027 6,the correlation coefficient r = 0. 999 9. The concentration of fluconazole in the lung tissue of group A was（ 28. 1 ± 19. 6） μg / ml,and in group B was（ 26. 6 ± 17. 7） μg / ml,the difference was not statistically significant（ P〈0. 05）. CONCLUSIONS： Ambroxol has no significant effect on concentration of fluconazole in the lung tissue,yet it still needs the further research combined with the clinical practice.
出处
《中国医院用药评价与分析》
2016年第3期366-368,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
常州市科技计划项目(NO.CS20109012)
