摘要
为查明秋石斛不同品种关键赋香成分,利用固相微萃取(SPME)方法结合GC-MS技术,测定了秋石斛5个具香气的品种,即绿天使(Dendrobium Hand Green)、日出2号(Dendrobium Burana Sunrise No.2)、白花607(Dendrobium K.B.White 607)、紫背256(Dendrobium Blue Sapphine 256)、魅力(Dendrobium Burana Charming)以及2个不具香气的品种,即红牛(Dendrobium Red Bull)、三亚阳光(Dendrobium Sunya Sunshine)盛花期的花朵挥发性成分及其相对含量。结果表明:共鉴定出45种挥发性化合物,其中萜烯类34种、芳香族化合物8种、酯类3种,5种具香气的秋石斛花朵所含挥发性成分绝大部分都是萜烯类,萜烯类对秋石斛的花香起着重要的作用。通过比较发现,5种具香气秋石斛的主要赋香成分为3-蒈烯、芳樟醇、α-可巴烯和α-法尼烯,不同品种挥发性成分的组成和含量明显不同。绿天使和日出2号的主要香气成分是3-蒈烯,相对含量分别为59.343%和77.775%,但日出2号中的释放率约为绿天使的3倍;白花607主要香气成分为3-蒈烯(29.170%)、α-可巴烯(17.660%)、芳樟醇(10.990%);紫背256中α-法尼烯相对含量最高(42.310%);魅力中主要香气成分是α-可巴烯(33.648%),邻苯二甲酸二异丁酯(13.866%)为其次。2个不具香气品种中鉴定出化合物较少,主要挥发性成分释放率较小;红牛主要挥发性成分是胡莫柳酯(28.118%),三亚阳光是异丁子香酚(27.529%)。这些主要挥发性成分对不同品种秋石斛花的特有香味起决定性作用,且大部分已被广泛应用于香精香料,医药,日化等产品中。该研究结果为香型秋石斛产品开发及品种的培育提供了参考。
In order to understand the volatile constituents and relative contents in full-blown flower of five aroma cuhi- vars （Dendrobium Hand Green, Dendrobium Burana Sunrise No.2, Dendrobium K. B. White 607, Dendrobium Blue Sapphine 256 and Dendrobium Burana Charming） and two non-fragrant cuhivars （Dendrobium Red Bull, and Dendrobium Sunya Sunshine） of Dendrobium, were deter-mined by solid-phase microextraction （SPME） and gas chroma- tography coupled with mass spectrometry （GC-MS）. The results showed that there were 45 volatiles identified in seven cultivars, including 34 kinds of terpene, 8 kinds of aromatic and 3 kinds of esters. Five scented varieties volatile com- ponents mostly were terpene, and the terpene played an important role in aroma formation of Dendrobium. By compari- son： main volatiles in five scented Dendrobium cuhivars were 3-carene, linalool and α-copaene. Different species＇ volatiles and relative contents were significantly distinct. The major components of Dendrobium Hand Green and Den- drobium Burana Sunrise No.2 were 3-carene, the relative contents were 59.343% and 77.775%, but the emission rate of Dendrobium Burana Sunrise No.2 was about 3 times of Dendrobium Hand Green＇ s; Dendrobium K. B. White 607＇ s main volatiles was 3-carene （ 29.170%） ,α-copaene （17.660%） , and linalool （ 10.990% ） ; In Dendrobium Blue Sap- phine 256＇s volatiles, the relative contents of α-farnesene was the highest （42.310%） ; the main volatiles in Den- drobium Burana Charming＇ s were α-eopaene （ 33.648% ） , followed by 1,2-benzenediearboxylic acid, his （ 2-methyl- propyl） ester （13.866%）. There were identified less volatiles in two non-fragrant cultivars, the main volatiles emission rates were also less; Dendrobium Red Bull＇s main volatile was homosalate （28.118% ） ; the most important volatile was isoeugenol （ 27.529% ） in Dendrobium Sunya Sunshine. The main volatile components played a decisive role of the scent in different varieties and most of them had been widely used in flavor, pharmaceutical, cosmetic and other prod- ucts. Therefore, looking for the key aroma components in different euhivars would provide references for aroma cultivar breeding and products development of Dendrobium.
出处
《广西植物》
CAS
CSCD
北大核心
2016年第3期361-368,288,共9页
Guihaia
基金
国家农业部“引进国际先进农业科学技术”重点项目(2011-413)
国家国际合作专项(2011DFB31690)
中央级公益性科研院所基本科研业务费专项(1630032015017,1630032014014)~~
关键词
秋石斛
挥发性成分
SPME
GC
MS
应用
Dendrobium, floral volatiles, solid-phase microextraction, GC-MS, application
