摘要
目的测定林下山参片中有机酸的含量。方法以食品中有机酸的含量测定标准GB/T 5009.157-2003,利用反相高效液相色谱法(RP-HPLC)测定林下山参片中富马酸、柠檬酸、马来酸、琥珀酸、酒石酸、香草酸和肉桂酸等7种有机酸的含量,采用XAqua C18色谱柱(4.6 mm×250 mm,5μm),以(NH4)2HPO4-H3PO4缓冲溶液(p H=2.7)为流动相分离测定前五种有机酸,流速为0.8 m L?min-1,柱温为30℃,检测波长210 nm;用Unitary C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.5%冰乙酸梯度洗脱测定香草酸和肉桂酸含量,流速为1.0 m L?min-1,柱温30℃,检测波长为260 nm。结果 :马来酸、柠檬酸、反丁烯二酸、香草酸及肉桂酸的含量分别为0.015‰、2.619‰、0.030‰、0.009‰、0.025‰。结论首次通过RP-HPLC法对林下山参片中的有机酸进行含量测定,为林下山参新产品的科学服用提供了依据。
Objective To determine the content of organic acid in tablets of Panax ginseng C.A.Meyer cv.Silvatica. Methods： According to GB/T 5009.157-2003, using the method of reversed phase high performance liquid chromatography（RP-HPLC） to determine the content of 7 kinds of organic acids like fumaric acid, citric acid, maleic acid, succinic acid, tartaric acid, vanillic acid and cinnamic acid in tablets of Panax ginseng C.A.Meyer cv. Silvatica. The former 5 kinds of organic acids were separated by XAqua C18 chromatographic column（4.6 mm×250 mm,5 μm）,（NH4）2HPO4-H3PO4 buffer solution（p H = 2.7） as mobile phase, flow velocity was 0.8 m L·min^-1, column temperature was 30℃, and the detection wavelength was 210 nm. The other two kinds of organic acids were separated by Unitary C18 chromatographic column（ 4.6mm×250 mm,5 μm）, methanol-0.5% ice acetic acid as mobile phase, gradient elution, flow velocity was 1.0 m L·min^-1, column temperature was 30℃, and the detection wavelength was 260 nm. Results Maleic acid, citric acid, fumaric acid,vanillic acid and cinnamic acid content were 0.015‰, 2.619‰, 0.030‰, 0.009‰, 0.025‰respectively. Conclusion： It is the first time to determine the contents of these kinds of organic acids by RP-HPLC method for the tablets of Panax ginseng C.A.Meyer cv. Silvatica, which provides a scientific basis for the development of new products.
出处
《人参研究》
2016年第1期6-8,共3页
Ginseng Research
关键词
林下山参片
有机酸
RP-HPLC
含量测定
Tablets of Panax ginseng C.A.Meyer cv
Silvatica
organic acid
RP-HPLC
Content determination
