摘要
目的:简化样品的前处理,避免高效液相色谱淋洗液乙腈、丙酮溶液对人体的损害,降低成本、提高仪器稳定性.方法:高效液相色谱法用 0.1% 甲酸的甲醇溶液作为淋洗液,不用梯度洗脱测定食品中苏丹红含量.结果:研究了用高效液相色谱法测定食品中苏丹红的色谱条件、线性范围,方法的检出限苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ分别为:11、10、8、8 μg/kg,相对标准偏差 <2.6%,回收率为 88%～106%,此方法与国标方法无显著性差异.结论:用本方法测定食品中苏丹红含量时有操作简单、快捷、毒性小、成本小、仪器稳定性好、灵敏度高等优点,具有可靠的准确度和良好的精密度.
Objective：To predigest former disposal of sample ,avoid the damage of eluent：acetonitrile and acetone to the hu- man body, reduce the cost and improve stability of HPLC apparatus. Methods： Sudan in food was determined by HPLC with eluent of methanol containing 0. 1% formic acid without grads elution. Results. The optimum chromatographic conditions, linear range were studied thoroughly. The detection limit of sudan Ⅰ , Ⅱ, Ⅲ, Ⅳ in food was 11,10,8,8 μg/kg respectively, RSD was less than 2. 6%, the rate of recovery ranged from 88% to 106%. There was no significant difference between the results by HPLC method and the results of national standard method. Conclusion：The determination of sudan in food by HPLC was easy and rapid to operate, less virulence, lower costs, good stability of apparatus, reliable accuracy and high precision. It also showed higher sensitivlty
出处
《中国卫生检验杂志》
CAS
2005年第11期1313-1315,共3页
Chinese Journal of Health Laboratory Technology
