摘要
目的观察胸腺肽对哮喘小鼠免疫功能及相关炎症因子的影响,初步探讨胸腺肽在哮喘小鼠治疗中的作用。方法选用40只清洁级BCLB雌性小鼠,采用鸡卵白蛋白(OVA)雾化吸入制作哮喘模型,然后将哮喘小鼠随机分成两组,即A组(基础治疗组)、B组(实验治疗组),另设健康对照组C组(空白对照组)20只。A组小鼠给予基础治疗,即雾化吸入糖皮质激素联合β受体激动剂,B组小鼠在基础治疗的同时联合胸腺肽皮下注射2.5 mg/kg,每周两次,连续应用12周。C组小鼠给予雾化生理盐水以对照。采用间接荧光法分别测定治疗前后哮喘小鼠血清中T细胞亚群CD3^+、CD4^+、CD8^+表达量变化;应用酶联免疫试验(ELISA)测定治疗前后哮喘小鼠血清中白细胞介素-4(IL-4)、白细胞介素-13(IL-13)、白细胞介素-12(IL-12)、IFN-γ的含量变化。结果治疗前A组和B组小鼠T细胞亚群CD3^+、CD4^+、CD4^+/CD8^+表达量较C组明显下降,CD8^+较C组升高,差异具有统计学意义(P<0.05),而A组和B组之间差异无统计学意义;治疗后A组和B组小鼠T细胞亚群CD3^+、CD4^+、CD4^+/CD8^+较治疗前表达量增加,差异具有统计学意义(P<0.05),B组较A组增加更明显,但两者之间差异无统计学意义(P>0.05)。治疗前A组和B组小鼠血清中IL-4和IL-13的水平高于C组(P<0.05),治疗后A组和B组血清中IL-4、IL-13表达量均有下降,但仍高于C组;而治疗前A组和B组小鼠血清中IFN-γ、IL-12、IFN-γ/IL-4、IL-12/IL-13较C组均有显著下降,差异具有统计学意义(P<0.05)。治疗后A组、B组血清中IFN-γ、IL-12、IFN-γ/IL-4、IL-12/IL-13表达量均较治疗前上升,且B组上升幅度高于A组,但两者间差异无统计学意义(P>0.05)。结论胸腺肽在调节Th1/Th2平衡方面起到一定增强作用,同时其对T细胞亚群的影响可能参与改善哮喘小鼠的免疫功能。
ObjectiveTo explore the effect of thymosin on immune function and related inflammatory factors of asthma mice, and preliminarily discuss the role of thymosin played in the treatment of asthma in mice.MethodsSerum was collected from sixty BALB/C female mice which were randomly divided into three groups： foundational treatment group （group A）, the experimental treatment group （group B） and healthy control group （group C）. Asthma model was established by inhaling chicken ovalbumin （OVA）, and group A of mice were given basic treatment, namely atomizing inhaled corticosteroids in combination with beta agonists. At the same time group B was given the basic treatment as well as usingnbsp;thymosin 2.5 mg/kg/subcutaneous injection, twice a week, continuous application of 12 weeks. While the C group was given the 0.9% saline instead. The expression of T cell subsets CD3＋, CD4＋, CD8＋ in serum of all mice before and after treatment was determined by indirect fluorescence. The level of IL-4, IL-13, IL-12 and IFN-γ in serum of all mice before and after treatment was determined by ELISA.ResultsCompared with group C, the expression of T cell subsets CD3＋, CD4＋, CD4＋/CD8 in serum of group A and B before treatment decreased obviously （P〈0.05）, while the CD8＋ expression increased significantly （P〈0.05）, but there was no statistically significant difference between group A and group B. Compared with group A and B before treatment respectively, the expression of T cell subsets CD3＋, CD4＋, CD4＋/CD8 in serum of group A and B after treatment increased obviously （P〈0.05）, and group B increased much obviously than group A, but there was no statistically significant difference between them （P〉0.05）. The level of IL-4 and IL-13 of the serum of group A and B were higher than group C before treatment, and the difference was statistically significant （P〈0.05）. Compared with group A and B before treatment, the level of IL-4 and IL-13 of the serum after treatment were decreased, which was also higher than group C. The level of IFN-γ, IL-12, IFN-γ/IL-4, IL-12/IL-13 of the serum of group A and B were lower than group C before treatment, and the difference was statistically significant （P〈0.05）. Compared with group A and B before treatment, the level of IFN-γ, IL-12, IFN-γ/IL-4, IL-12/IL-13 of the serum after treatment were increased, and the level of them were increased remarkably in group B, but the difference between group A and B was no statistically significant （P〉0.05）.ConclusionsThymosin played a role in regulating the balance of Th1/Th2, and may improve immune function of asthma mice by effects on T cell subgroup.
出处
《中华临床医师杂志（电子版）》
CAS
2016年第6期808-812,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
山东省科技攻关课题(2012GSF11830)
山东省自然科学基金(ZR2013HM046)
关键词
胸腺肽
哮喘
免疫功能
炎症因子
Thymosin
Asthma
Immune function
Inflammatory cytokines
