摘要
为了经济高效地利用餐厨垃圾发酵产微生物油脂,采用杯罐实验对餐厨垃圾原液进行热预处理、酸碱预处理和氧条件预处理,以探求酿酒酵母菌S.cerevisiae发酵产微生物油脂的最佳预处理条件。试验结果表明,餐厨垃圾原液热预处理的最佳温度为60℃,60℃温度下预处理2h后,餐厨垃圾水解液中的还原糖百分数和氨基酸态氮含量达到最大,分别为3.780 4%和0.539 mg/mL;pH值为[2,11]的酸碱预处理中,pH值为8时效果最佳,此条件下还原糖百分数和氨基酸态氮含量分别为3.210 4%和0.889mg/mL;相对于好氧条件,餐厨垃圾原液在兼氧条件下处理24h还原糖百分数和氨基酸态氮含量均较大,其值分别为3.221%和0.56mg/mL。在试验获得的最佳预处理条件下,采用Saccharomyces cerevisiae As2.516作为供试菌株,以预处理后的餐厨垃圾水解液加入量90%(V/V)、搅拌速率150r/min、通气量2.5L/min、发酵周期7d为基础发酵条件,进行1L发酵罐发酵,获得油脂产率为26.86%。
In order to utilize the microbial oil production in fermentation of hydrolysis liquid of kitchen garbage economically and efficiently,heat pretreatment,acid and alkali pretreatment,and oxygen content pretreatment in different conditions was performed for the raw liquor to find the best pretreatment conditions of producing microbial oil by fermentation of Saccharomyces cerevisiae As2.516.The results showed that the optimum temperature of heat pretreatment was 60℃,the percentage of reducing sugar and the content of amino acid nitrogen in the hydrolysis liquid of kitchen garbage after two-hour pretreatment at 60 ℃peaked to 3.780 4% and 0.539 mg/mL respectively.In the acid and alkali pretreatment at pH value 2to 11,the two indicators witnessed the highest were 3.210 4%,0.889 mg/mL respectively when the pH value of the initial liquid was 8.Compared to aerobiotic condition,the percentage of reducing sugar and the content of amino acid nitrogen of the raw liquor of kitchen garbage which was pretreated 24 hours in the facultative anaerobe condition were higher,achieving 3.221%,0.56 mg/mL respectively.The Saccharomyces cerevisiae As2.516 was placed in a 1Lfermentator to ferment under the optimum conditions acquired from the experiment-the initial addition of hydrolysis liquid of kitchen garbage 90%（V/V）,the agitation speed 150r/min,aeration rate 2.5L/min and the fermentation cycle 7d,and the oil production was high as 26.86%.
出处
《长沙理工大学学报（自然科学版）》
CAS
2016年第1期99-104,共6页
Journal of Changsha University of Science and Technology:Natural Science
基金
国家自然科学基金资助项目(51309032
51408068
51308069)
关键词
餐厨垃圾
预处理
发酵
微生物油脂
kitchen garbage
pretreatment
ferment
microbial oil
