摘要
从罹病的玉米螟（Ostrinia furnacalis G）中分离纯化获得一株新菌株,命名为Bb-1。通过对该菌株的培养性状、形态特征观察,以及ITS基因序列分析,鉴定出Bb-1菌株为白僵菌属的球孢白僵菌（Beauveria bassiana）。用该菌株的5种浓度孢子液对黄胸散白蚁进行毒力试验。结果表明：用浓度为5×10^7孢子/mL的孢子液处理8d,黄胸散白蚁的校正死亡率达95.00%。5-8d的LC50值为2.05×10^4-9.05×10^6孢子/mL。在5×10^3-5×10^7孢子/mL浓度下,菌株的LT50值为4.28-8.59d。
A new strain of Beauveria bassiana designated No. Bb-1 was isolated and purified from the diseased larvae of Ostrinia furnacalis G. The strain was identified bases on its culture traits,morphological characteristics,and ITS sequence analysis. The results showed that the strain belonged to Beauveria bassiana. In virulence test,the corrected mortality rate reached 95.00% under the concentration of 5×10^7 spores/mL. The values of LC50 were 2.05×10^4-9.05×10^6 spores/mL. Under different concentrations,the LT50 values were 4.28-8.59 d.
出处
《现代农药》
CAS
2016年第2期20-23,共4页
MODERN AGROCHEMICALS
基金
国家自然科学基金项目(31272106)
山东省“泰山学者”建设工程专项
关键词
白僵菌
Bb-1
黄胸散白蚁
鉴定
毒力
Beauveria bassiana
Bb-1
Reticulitermes flaviceps
identification
virulence
