摘要
从火龙果果实表面上筛选出一株发酵产γ-氨基丁酸(GABA)白色菌株,经形态学观察、生理生化试验和18S rDNA测序分析,鉴定为假丝酵母菌菌株(Candida.sp),命名为C2。C2作为出发菌株,分别采用紫外线(UV)和亚硝基胍(NTG)诱变方法选育高产γ-氨基丁酸菌株。与出发菌株相比,紫外诱变菌株γ-氨基丁酸产量增加了40.25%,亚硝基胍诱变菌株γ-氨基丁酸产量增加了62.83%。通过紫外线和亚硝基胍复合诱变,得到正向突变株,其中Y6突变株遗传性状稳定,γ-氨基丁酸产量达2.561 g/L,产量比诱变前提高了3.1倍。
White strains producing γ-aminobutyric acid（GABA） were screening from the surface of pitaya fruits. Strains named C2 were identified as Candida.sp by morphological observation,physiological and biochemical characteristics and 18 Sr DNA sequence analysis.C2 as the starting strain was screened by UV and NTG to get high-yield of γ-aminobutyric acid yeast. Compared with the starting strain,the data showed that the increasing rate of γ-aminobutyric acid production was 40.25 % from a strain by UV mutagenesis and 62.83 %from a strain by NTG mutagenesis.Y6 with stable γ-aminobutyric acid production was obtainded by UV combined with NTG.The γ-aminobutyric acid production from Y6 was 2.561 g/L and increased about 3.1 times than that before mutagenesis.
出处
《食品研究与开发》
CAS
北大核心
2016年第2期177-182,共6页
Food Research and Development
