摘要
目的:建立RP-HPLC同步测定延胡索中11种生物碱成分的方法,为该药材的质量控制提供参考。方法:Kromasil Eternity C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.1 mol·L-1乙酸铵溶液(每1 L溶液中加冰乙酸0.5m L)(B)梯度洗脱(0~50 min,20%~55%A;50~55 min,55%~65%A;55~60 min,65%A),流速1.0 m L·min-1,柱温25℃,检测波长280 nm,进样量10μL。结果:11种待测成分的线性关系良好,相关系数均>0.999;精密度试验RSD 0.1%~2.0%,稳定性试验RSD 0.2%~2.1%,重复性试验RSD 1.7%~4.1%,平均加样回收率98.2%~100.8%(RSD 1.6%~3.5%),均符合中药质量分析要求。结论:建立的RP-HPLC灵敏、简便、可靠,可用于延胡索药材中多种生物碱成分的同步测定。
Objective： An efficient method using RP-HPLC was developed for simultaneous determination of eleven major active alkaloids in Corydalis Rhizoma. Method： Chromatographic separation was achieved on a Kromasil Eternity C18column（ 4. 6 mm × 250 mm,5 μm） with a gradient mobile phase of acetonitrile-0. 1 mol·L^（- 1）ammonium acetate solution（ added 0. 5 m L of glacial acetic acid per 1 L） at a flow rate of 1. 0 m L · min- 1,detection wavelength was set at 280 nm and column temperature was at 25 ℃. Result：Eleven major alkaloids showed a good linearity with r〉 0. 999 and good precision（ RSD of 0. 1%-2. 0%）,stability（ RSD of 0. 2%-2. 1%）,repeatability（ RSD of 1. 7%-4. 1%）; average recovery was 98. 2%-100. 8%with RSD of 1. 6%-3. 5%. Conclusion： This established method is sensitive, convenient and reliable for determining eleven major components in Corydalis Rhizoma.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第6期22-25,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药行业科研专项(201407003)
北京市自然科学基金项目(7132152)
