摘要
为探究马铃薯(Solanum tuberosum)不同遗传特性与其对水分胁迫响应之间的相关关系,本研究通过SSR分析了供试材料的遗传多样性,并通过PEG-6000和不浇水胁迫处理马铃薯移栽苗的方法,研究了干旱胁迫下不同马铃薯品种中过氧化物酶(POD、CAT)活性及可溶性蛋白含量的变化。结果显示,在遗传相似系数(GS)为0.700水平上,44份马铃薯材料可被聚为5类,大部分品种聚在一起,说明供试品种的遗传基础相对狭窄。通过GGE-Biplot综合分析POD、CAT及可溶性蛋白质变化量,将44份供试材料分为6组。比对分析遗传多样性分析与生理结果,发现二者之间并不具有明显的一致性,即品种遗传特性的差异并不能直接反映品种的抗旱性能差异,而两种胁迫方式在研究品种抗旱特性上具有较高的一致性。该研究结果可以为今后抗旱试验的进行提供参考。
To explore the relationship between potato（Solanum tuberosum）different genetic traits and its response to water stress,we analyzed the genetic diversity of test materials by the methods of SSR,studied the content variation of peroxidase（POD and CAT）activity and soluble protein of different potato varieties under water stresses by processing potato transplanting seedling for PEG-6000 simulated water stress and natural water stress method.The results showed that the genetic similarity coefficient（GS）at 0.700 level,44potato materials can be divided into five types,most breed clustered together,explaining the genetic basis of testing varieties is still relatively narrow.Through a GGE-biplot comprehensive analysis of soluble protein,POD and CAT variation,44 selected materials were divided into 6groups.Comparative analysis of genetic diversity analysis and physiological results found that there were no obvious consistency between them,namely the species differences in genetic traits can＇t react their drought resistance performance difference directly.But two stress modes with high consistency on studying the characteristics of varieties of drought resistance.This research provides the reference for further drought resistance research.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第3期431-441,共11页
Pratacultural Science
基金
科技部国际科技合作项目(2014DFG31570)
甘肃科技基金(1308RJZA131
1308RJIA005)
兰州科技研究项目(2013-4-156)
关键词
遗传多样性
PEG胁迫
双标图
抗旱性
genetic diversity
PEG stress
GGE-biplot
drought resistance
