摘要
背景:大鼠脊髓完全性横断模型是研究神经组织工程的常用模型,使用既往造模方法横断脊髓后,无法保证断端间隙长度的相对统一,以致无法客观评价各种治疗方法或组织工程材料的效果。目的:采用自制的双刃显微剪刀建立大鼠脊髓完全横断缺损模型,并通过与常规造模方法比较,探索这种新模型的可行性。方法:成年雌性SD大鼠42只,随机分为A组(n=6)、B组(n=18)和C组(n=18),A组仅行椎板切除;B组用尖刀在已显露脊髓的中间位置横向切断脊髓,刀尖要触及椎管前壁和侧壁骨面,反复切割,制备脊髓完全横断模型;C组采用自制双刃显微剪刀制备脊髓完全横断模型,用一钝头小钩将手术缝线从已显露脊髓的腹侧穿到对侧,轻微抬起脊髓,用双刃显微剪刀将脊髓、硬脊膜及血管一次性全部切除。结果与结论:1B和C组大鼠造模后1周,后肢完全性瘫痪,BBB评分接近,但脊髓残端间距离有显著差异。2造模后4周,两组大鼠后肢功能有不同程度恢复,但BBB评分差异无显著性意义。3造模后8周,两组大鼠后肢运动功能评分差异有显著性意义;生物素化葡萄糖胺示踪显示:B组大鼠损伤尾侧可观察到少量被标记的轴突纤维,而C组大鼠尾侧无被标记的神经纤维,说明脊髓横断完全。4以上结果提示:使用自制的双刃显微剪刀造模方法可有效消除个体差异,有利于治疗效果的量化分析和研究对比。
BACKGROUND： Rat models of complete spinal cord transection are common models for neural tissue engineering. After transecting the spinal cord by the previous methods, gap length of broken end cannot keep relatively uniform, so we cannot objectively evaluate effects of various treatments or tissue engineering materials. OBJECTIVE： The spinal cord transection models were established by using double edged micro scissors, andthe feasibility of this new model was explored by comparing with the conventional method. METHODS： A total of 42 adult female Sprague-Dawley rats were divided randomly into group A（n=6）, group B（n=18） and group C（n=18）. Group A only received laminectomy. In the group B, the spinal cord was transected with a sharp-pointed knife. Knife point should touch anterior wall of spinal canal and sidewall bone surface. Complete spinal cord transection models were prepared by repeated cutting. In group C, complete spinal cord transection models were established by using self-made double edged micro scissors. RESULTS AND CONCLUSION：（1） At 1 week after model establishment, in the groups B and C, complete paralysis of the hind limbs was found, and BBB scores were similar. However, significant differences in the spacing of broken end were detected.（2） At 4 weeks after model establishment, hind limb functions could restore to different degrees in both groups, but no significant difference in BBB scores was found.（3） At 8 weeks after model establishment, significant differences in hindlimb motor function scores were detectable between both groups. Biotin glucosamine tracer display： In group B, a few labeled axon fibers were observed at the caudal side of the injured spinal cord. In group C, spinal cord was completely transected, and labeled axon fibers cannot be found at the caudal side.（4） Results suggested that the modeling method of self-made double edged micro scissors could effectively eliminate individual differences, contribute to quantitative analysis and comparative study of therapeutic effects.
出处
《中国组织工程研究》
CAS
北大核心
2016年第5期635-639,共5页
Chinese Journal of Tissue Engineering Research
基金
教育部2012年度"新世纪优秀人才支持计划"(NCET-12-0805)~~
