摘要
目的探讨脂多糖(LPS)对星形胶质细胞的活化作用及内向整流性钾离子通道4.1(Kir4.1)表达的影响。方法分离培养新生SD大鼠大脑皮层星形胶质细胞;LPS处理或者和白细胞介素1受体拮抗剂(IL-1ra)处理培养的细胞,MTT法检测细胞活力,免疫细胞化学技术检测星形细胞胶质纤维酸性蛋白(GFAP)的表达,ELISA检测细胞培养上清中的IL-1β的水平,实时定量PCR法检测星形胶质细胞IL-1β和Kir4.1 mRNA的表达情况。结果 LPS促进星形胶质细胞的活化具有浓度和时间依赖性。LPS可促进星形胶质细胞IL-1β的分泌和IL-1βmRNA的表达,下调Kir4.1 mRNA的表达;与LPS组相比较,IL-1ra可以有效对抗上述两结果。结论 LPS可诱导培养的星形胶质细胞活化;LPS下调星形胶质细胞Kir4.1 mRNA表达可能与IL-1β有关。
Objective To explore the influence of lipopolysaccharide（ LPS） on the activation of astrocytes and the expression of the inwardly rectifying K＋channel Kir4. 1. Methods Astrocytes were separated from the cerebral cortex of newborn SD rats and cultured in the presence of LPS or LPS combined with interleukin-1 receptor antagonist（ IL-1ra）. The cell vitality was detected by MTT assay; the expression of glial fibrillary acidic protein（ GFAP） was analyzed by immunocytochemistry; the production of IL-1β was tested by ELISA; the expression of Kir4. 1 and IL-1β mRNAs were determined by quantitative real-time PCR. Results LPS promoted the activation of astrocytes in a time-and dose-dependent manner. LPS significantly increased the production of IL-1β and the expression of IL-1β mRNA,while decreased the expression of Kir4. 1mRNA in cultured astrocytes. Compared with the LPS group,IL-1ra could effectively reversed the above two results.Conclusion The cultured astrocytes could be activated by LPS; LPS-induced downregulation of Kir4. 1 mRNA in cultured astrocytes might be related with the inflammatory cytokine IL-1β.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2016年第2期196-200,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81171117)
教育部留学回国人员科研启动基金(2012年)
安徽科技计划项目(1506c085014)
