摘要
本研究确定伐昔洛韦的体外和体内抗猪伪狂犬病病毒作用,确定伐昔洛韦对Vero细胞的最大无毒质量浓度为4g/L,抑制PRV野毒在Vero细胞中复制的最低有效质量浓度为3g/L。用1个LD50的PRV对小鼠进行攻毒,在攻毒后48h给服不同剂量的伐昔洛韦,确定了伐昔洛韦能够为小鼠提供完全保护的最低剂量为3 mg/只。PRV攻毒后,未给服伐昔洛韦组小鼠表现典型的神经症状并全部死亡,而给药组小鼠临床健康并全部存活。与给药组小鼠相比,未给药组小鼠脑、肺组织中的PRV病毒载量明显高于给药组小鼠,且随时间延长一直增加。脑、肺组织呈现PRV感染的特征性病理变化。伐昔洛韦给药组小鼠相应组织中的病毒载量和脾脏T淋巴细胞分泌PRV特异性Th1型细胞因子水平在攻毒后72h逐渐降低。试验结果显示,伐昔洛韦无论在体内、体外均能够有效抑制PRV的复制。
In this research, anti-porcine pseudorabies virus （PRV） effects of valacyclovir in vitro and in vivo were investigated, maximum non-toxic concentration of valacyclovir to Vero cells and minimal effective concentration of valacyelovir for inhibiting of replication of PRV were determined as 4 and 3 g/L,respectively. Mice were challenged with 1 LDS0 PRV,and were given different dosages of valacyclovir orally at 48 h post-challenge. The lowest protective dose of valacyclovir against 1 LDS0 PRV challenge was 3 rag/mouse. After PRV challenge,mice not given valacyclovir developed typical neural symptoms and died, however mice given valacyclovir remained clinical health and alive. PRV viral loads in brain and lung tissues of mice not given valacyclovir were much higher than those of mice given valaciclovir. Brain and lung tissues of mice not given valacyclovir developed typical lesions, however brain and lung tissues of mice given valacyclovir remained normal. Results in this study demonstrated that valacyclovir could inhibit replication of PRV in vitro and in vivo effectively.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2016年第2期298-304,共7页
Chinese Journal of Veterinary Science
基金
河南省高校科技创新团队支持计划资助项目(14IRTSTHN015)
关键词
猪伪狂犬病病毒
复制
伐昔洛韦
抑制
porcine pseudorabies virus
replication
valacyclovir
inhibition
