摘要
为探明沙门氏菌生物菌膜的结构特征及其在食品加工接触表面的转移率,以TSB为标准生长基质,研究了沙门氏菌在TSB和肉质液(MTLB)2种培养基质中形成的生物菌膜结构特征,并利用"夹心粘附法"测定2种生物菌膜在常见的5种食品加工接触材料表面的转移率。结果表明:沙门氏菌在TSB中的生物菌膜形成数量显著大于MTLB;激光共聚焦扫描显微镜(CLSM)结果显示,沙门氏菌在MTLB中形成的生物菌膜结构特征偏向于片状聚集的云雾状结构,而在TSB中则呈现密集分布的网状结构;扫描电子显微镜(SEM)结果表明,2种基质中所形成的生物菌膜微观聚集体结构无明显差别;2种生物菌膜在5种食品接触面的转移率存在显著差异,MTLB中的生物菌膜在不锈钢和玻璃表面的转移率较高,TSB中的生物菌膜在聚乙烯表面的转移率较大。本研究可为沙门氏菌风险评估提供基础数据,为改进沙门氏菌的清洗控制措施提供参考。
In order to determine the microstructer of Salmonella biofilm and its transfer to food-processing surfaces. As compared to a standard growth media（ TSB）,a meat thawing-loss broth（ MTLB） was used as a growth culture in biofilm formation. Based on evaluation of the microstructer of biofilm in the two growth culture,the ‘sandwich-transfer’method was used to test the transfer rates of biofilm from stainless steel surfaces to other 5 food processing surfaces. The results showed that the cells number in biofilm grown in TSB were greater than that in biofilm grown in MTLB. The confocal laser scanning microscopy（ CLSM） results showed that Salmonella grown in MTLB showed ‘cloud-shaped’morphology in the mature biofilm,whereas when grown in TSB appeared ‘reticular-shaped’. However,there was no significant difference in microstructer of biofilm when grown in the two different media,confirmed by scanning electron microscopy（ SEM）. The transfer rates（ RTs） of biofilm were significantly influenced by the types of processing surfaces,with stainless steel and glass surface showing higher RTs in MTLB biofilm,and polyethylene surface showing higher RTs in TSB biofilm. The study could provide useful data in quantitative microbiological risk assessment of Salmonella,and supply information in improving the control measures of Salmonella.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第12期2313-2319,共7页
Journal of Nuclear Agricultural Sciences
基金
江苏省科技计划青年基金项目(BK20150678)
国家自然基金青年基金项目(31501574)
