摘要
目的探讨细胞块免疫组化技术在恶性浆膜腔积液组织来源及分子病理检测中的应用价值。方法抽取42例患者的恶性浆膜腔积液,分层离心获取肿瘤细胞并制作成细胞蜡块,应用HE染色、免疫组化染色、基因测序等方法对其进行检测。结果 42例患者中,利用恶性浆膜腔积液细胞块明确组织分型及组织来源40例,其中肺癌29例(腺癌24例、鳞癌3例、小细胞癌2例),恶性淋巴瘤2例,胃肠道癌4例,乳腺癌4例,卵巢癌1例。29例肺癌样本中24例进行了肺腺癌基因检测,其中9例表皮生长因子受体基因突变,包括E19(del E746-A750)缺失突变6例和E21(L858R)替代突变3例。结论细胞块技术对于恶性浆膜腔积液的诊断有重要临床意义,有助于明确诊断并查找组织来源,同时能够进行组织分型及分子病理检测,有效指导肿瘤的分子靶向治疗。
Objective Application of cell block from malignant serous cavity effusion in histopathological and molecular pathological examinations. Methods Forty-two samples of malignant serous cavity effusion were centrifugalized and the tumor cells were obtained for cell block, the hematoxylin and eosin （HE） staining, immunohistochemical staining, gene sequencing were used for investigation. Results Among 42 cell blocks, the histological types and tissue sources were confirmed in 40, including 29 cases of lung cancer （24 adenocarcinoma, 3 squamous cell carcinomas and 2 small cell carcinoma）, 2 cases of malignant lymphoma, 4 cases of gastrointestinal carcinoma, 4 cases of breast cancer and 1 case of ovarian cancer. Twenty four specimens of lung adenocarcinoma were subjected to genetic tests and 9 were found with EGFR gene mutations, including 6 cases of E19 （del E746-A750） and 3 cases of E21 （L858R）. Conclusion Serous cavity effusion cell block technique is of value in histopathological diagnosis and tumor targeted therapy.
出处
《浙江医学》
CAS
2015年第23期1891-1894,共4页
Zhejiang Medical Journal
基金
金华市科学技术研究计划项目(2014-3-121)
关键词
细胞块
组织来源
基因突变
Cell block Source of organization Genetic mutations
