摘要
目的:探讨一组鉴别浆膜腔积液中转移性腺癌原发部位的抗体。方法:对31例浆膜腔积液转移性腺癌制备细胞蜡块切片作TTF-1、CA125、CK7、CK20免疫细胞化学染色,这些病例经组织学或结合临床资料证实:肺腺癌15例、卵巢腺癌10例、胃肠癌6例。结果:肺腺癌的阳性表达分别是TTF-1(73.3%)、CA125(26.7%)、CK7(73.3%)、CK20(0%),卵巢腺癌的阳性表达分别是TTF-1(0%)、CA125(70.0%)、CK7(100.0%)、CK20(0%),胃肠腺癌的阳性表达分别是TTF-1(0%)、CA125(16.7%)、CK7(0%)、CK20(100.0%)。TTF-1在肺腺癌的表达具有高度敏感性(73.3%)及特异性(100%),CA125在卵巢腺癌的表达也有较高的敏感性(70.0%),CK7(-)/CK20(+)在胃肠腺癌的表达100%。结论:联合检测TTF-1、CA125、CK7、CK20能有效鉴别浆膜腔积液中来自肺、卵巢、胃肠道的腺癌。
Objective： To investigae a panel of immunohistochemical stains to help differentiate from the primary sites of metastatic adenocarcinoma in serous effusions.Methods： Thirty-one cases of metastatic adenocarcinoma in serous effusions were confirmed by histologic diagnoses and/or clinical,including 15 adenocarcinoma of lung,10 adenocarcinoma of ovary,6 gastrointestinal adenocarcinoma.Paraffin-embedded cell block sections were immunostained for TTF-1,CA125,CK7 and CK20.Results： The positive expression of lung adenocarcinoma were TTF-1（73.3%）,CA125（26.7%）,CK7（73.3%）,CK20（0%）;Ovarian adenocarcinoma were positive for TTF-1（0%）,CA125（70.0%）,CK7（100.0%）,CK20（0%）,gastrointestinal adenocarcinoma were positive for TTF-1（0%）,CA125（16.7%）,CK7（0%）,CK20（100.0%）.TTF-1 expression in lung adenocarcinoma with high sensitivity and specificity of 73.3%,100%,CA125 expression in ovarian adenocarcinoma had a higher sensitivity of 70.0%,the combination of CK7 negativity/CK20 positivity was seen in 100% of gastrointestinal adenocarcinoma.Conclusion： The combination of TTF-1,CA125,CK7 and CK20 stains is useful in distinguishing metastatic adenocarcinoma in serous effusions from lung,ovary and gastrointestinal.
出处
《现代肿瘤医学》
CAS
2012年第4期822-824,共3页
Journal of Modern Oncology
关键词
免疫细胞化学
转移性腺癌
浆膜腔积液
细胞块
immunocytochemical
metastatic adenodarcinomas
serous cavity effusions
cell blook
