摘要
以当年生野生山杏成熟种子为试材,采用组织培养法,研究了种皮、不同浓度赤霉素(GA_3)以及光照条件对山杏种子萌发的影响。结果表明:光照16h、黑暗8h条件下,MS培养基中添加0.16mg/L GA_3能有效促进去种皮山杏种子的萌发,未去除种皮的山杏种子均未萌发;在遮光条件下,去种皮种子在添加了0.16mg/L与0.50mg/L GA_3的MS培养基中发芽率均为100%,幼苗比光照16h、黑暗8h培养条件下的高且生长速度快,但黄化现象严重;将幼苗移至土壤中,能够正常生长,成活率达100%。
The mature seeds of prunus armeniaca was used as material to study the influence factors of embryo culture,including the seed testa,shading conditions and different concentration GA_3.The results showed that the MS medium added 0.16mg/L of GA_3 could increase the germination of embryo removed testa under light period 16h/8h.Under the shading condition,the germination rate of embryo removed testa was 100%in MS medium added of 0.16mg/L and0.50mg/L of GA_3.The seedlings growth was faster than that of under light period 16h/8h,but the etiolation was obvious.The seedlings which were transplanted to soil were normal with rival rate 100%.
出处
《北方园艺》
CAS
北大核心
2016年第3期106-108,共3页
Northern Horticulture
基金
承德市财政局资助项目(CZ2014004)
关键词
山杏
胚培养
GA3
Prunus armeniaca
embryo culture
GA_3
