摘要
为建立检测副溶血弧菌(Vibrio parahaemolyticus,VP)的快速检测方法,本研究以VPtoxR基因为靶基因设计合成引物及TaqMan探针,建立了实时荧光定量PCR快速检测VP的方法。结果显示,对15株试验菌株进行实时荧光定量PCR检测,只有VP检测为阳性,表明该检测方法特异性强;该方法的灵敏度为4.9CFU/mL,利用该检测方法对采集的150份样品进行检测,共计检出3份VP阳性样品,与国标法(GB 4789.7—2013)检测结果一致,显示了良好的实用性。该检测方法灵敏度高、特异性强,具有良好的实用性。
To establish a rapid assay for detection of Vibrio parahaemolyticus（VP）,Real-time PCR method was developed targeting to toxR gene of Vibrio parahaemolyticus.The results showed that the test for 15 bacteria strains using the Real-time PCR method,only Vibrio parahaemolyticus test was positive,indicating that the method had high specificity.In addition,the sensitivity of Real-time PCR was 4.9CFU/mL.Furthermore,a total of 3positive samples for Vibrio parahaemolyticus were detected from 150 clinical samples by the Real-time method,which was in accordance with the testing result by GB 4789.7-2013 standard detection protocol.Therefore,the Real-time method provided a novel rapid and sensitive detection method with good practicality for Vibrio parahaemolyticus infection.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第1期58-62,共5页
China Animal Husbandry & Veterinary Medicine
基金
海南省社会发展科技专项(2015SF29)
国家质检总局科技项目(2013IK031、2013IK051、2015IK089)
重庆市科技计划项目(cstc2014yykfA80017)
海南省应用技术研究与开发专项项目(ZDXM20130025)
