摘要
为了确定广西南宁纤维板霉变微生物的种类,从广西高峰人造板有限公司已霉变纤维板上采集霉变样品,经分离纯化,采用传统的形态学方法与ITS序列分析法相结合,进行了微生物的鉴定。分离筛选得到4株霉变真菌,编号为GXB-1,ZXB-2,ZXB-3和ZXB-4。根据传统形态学方法将4株霉变真菌鉴定为木霉属(Trichoderma sp.)和脉孢霉属(Neurospora sp.),其中木霉属真菌为优势种群,分离率高达60%。随后对4株霉变真菌进行r DNA-ITS序列分析和同源性比较,综合形态学鉴定结果,将菌株GXB-1鉴定为哈茨木霉,将菌株ZXB-2和ZXB-3鉴定为深绿木霉(T.atroviride),将菌株ZXB-4鉴定为四孢脉孢霉(N.tetrasperma XF35)。不同地区、不同基质和环境条件下,霉变微生物的优势菌种类存在较大的差异,这一结果可为筛选适宜的纤维板防霉剂提供参考。
In order to identify the microorganisms causing mildew of fiberboard in Guangxi, moldy fiberboard sampleswere collected as test objects from Gaofeng artificial board Co. LTD, Guangxi. After isolation and purification of mi-crobes, traditional morphological method and ITS sequences analyses were used to identify the microorganisms. 4 fungistrains were isolated from the samples, numbered as GXB-1, ZXB-2, ZXB-3 and ZXB-4, respectively. According tothe traditional morphological results, these strains were belonged to Trichoderma sip. and Neurospora sp.. Trichoderma sp.was the dominant population and the isolation rate was 60%. rDNA-ITS analysis and homology comparison were thenused to identify these strains. Phylogenetic tree showed that strain GXB-1 existed a common bunch with T. harzianumMGQ2（ GenBank accession number： KC342029. 1） , strain ZXB-2 and ZXB-3 were on the same bunch of T. viride andT. atroviride, strain ZXB- 4 existed a common bunch with N. tetrasperma XF35 （GenBank accession number：JX136749. 1）, which represented a similarity up to 99%. The comprehensive identification results showed that strainGXB-1 was T. harzianum, strain ZXB-2 and ZXB-3 were T. atroviride, strain ZXB-4 was N. tetrasperma, respective-ly. Dominant population of mildew strains were different in different area, matrix and environmental condition. This studycan provide a basis for selecting suitable anti-mildew agent for fiberboard.
出处
《林业工程学报》
北大核心
2016年第1期78-82,共5页
Journal of Forestry Engineering
基金
广西科技厅科技攻关项目(桂科攻14122005-36)
关键词
纤维板
霉变
ITS
鉴定
fiberboard
mildew
ITS
identification
